STED nanoscopy of actin dynamics in synapses deep inside
living brain slices.
Li Gan, PhD, hopes to use the microscope to image firing cells in
live brain slices, while Leor Weinberger, PhD, is interested in studying the processes by which a virus enters a cell in real time.
Not exact matches
These findings were confirmed by two - photon imaging of neurons in the
brains of
living mice by the lab of collaborator Yi Zuo, PhD, a neuroscientist at UC Santa Cruz, as well as electrophysiological recordings from neurons in
brain slices by the lab of collaborator Vikaas Sohal, MD, PhD, an associate professor of psychiatry at UCSF.
Another Kavli researcher, Steven Siegelbaum, uses a technology called two - photon microscopy to image pre-synaptic terminals (the nerve cell tips that send charges from one cell to another) within
slices of
living brain tissue.
In the Finkbeiner laboratory, Linsley uses time - lapse imaging of neurons in culture, within organotypic mouse
brain slices, and within
live zebrafish to uncover the mechanisms in neurodegeneration that lead to neuronal death.
Still, testing
brain slices might not represent what happens in the
brain of a
living animal.
1 - 4 days following DNA injection and electroporation, embryonic
brains are dissected,
sliced, placed on a filter in culture medium and
live imaged over night (Baffet et al., Methods Cell Biol., 2015).
My Italian - Canadian stepfather often ambushes me with
slice - o -
life zingers that rattle in my
brain for years to come.