AmpliSeq HiQ libraries for
sequencing were prepared on the Ion OneTouch 2 system, quantified using the Qubit 2.0 fluorometer (Life Technologies, Thermo Fisher Scientific, Waltham, MA, USA) and massive parallel
sequencing (MPS) was subsequently performed on the Ion Proton System utilizing a PI chip (Life Technologies, Thermo Fisher Scientific, Waltham, MA, USA), with 2 or 3 samples (in cases of family trio)
loaded per
run.