Sentences with phrase «localization microscopy»

«Ultra-high resolution imaging by fluorescence photoactivation localization microscopy.»

Indeed, within the world of super resolution microscopy, PA - FPs have helped create several new imaging methods, including photoactivation - localization microscopy (PALM, also FPALM)(11).

Furthermore, the microscope will be capable of performing live - cell super-resolution imaging through structured illumination microscopy (SIM) and Super-Resolution Radial Fluctuations (SRRF); for fixed cells resolutions on the scale of tens of nanometres will be achievable using single molecule localization microscopy (SMLM) techniques.

Motion model ultrasound localization microscopy (mULM) is a new tool using CEUS to morphologically and functionally characterize fine vascular networks in tumors at super-resolution.

(a, b) Single molecule localization microscopy.

By tracking a limited ensemble of diffusible molecules over time, the spatiotemporal dynamics of molecules and the connectivity within microcompartments can be obtained in addition to superresolved (tracking and localization microscopy, TALM).

We will use high and super-resolution fluorescence microscopy like total internal reflection fluorescence microscopy (TIRFM) and fluorescence photo - activation localization microscopy (FPALM) to visualize and track the spatio - temporal dynamics of tethering and SNARE proteins in live and fixed cells with single molecule resolution.

We demonstrated the technique on 20 different biological processes spanning four orders of magnitude in space and time, including the binding kinetics of single Sox2 transcription factor molecules, 3D superresolution photoactivated localization microscopy of nuclear lamins, dynamic organelle rearrangements and 3D tracking of microtubule plus ends during mitosis, neutrophil motility in a collagen mesh, and subcellular protein localization and dynamics during embryogenesis in Caenorhabditis elegans and Drosophila melanogaster.

But he notes that SIM has always offered two advantages over alternative super-resolution methods, including photoactivated localization microscopy (PALM), which he developed in 2006 with Janelia colleague Harald Hess.

The new technique, dubbed photoactivated localization microscopy, currently has a resolution similar to that of electron microscopy.

Photoactivated localization microscopy incorporates into a sample special fluorescent proteins that can be toggled between on and off states when hit with a particular wavelength of light.

Betzig and Moerner, working separately, developed a different method known as single - molecule localization microscopy.

Not exact matches

The focus is on protein detection in live versus fixed cells: determination of protein expression, localization, activity state, and the possibility for combination of fluorescent light microscopy with electron microscopy.

Through the use of localization - based super-resolution microscopy, researchers are able to photoactivate, image and follow these genetically encoded fluorescent proteins, one at a time, to study what is happening inside a cell at the molecular level.

Immunofluorescence microscopy for localization of Arabidopsis chloroplast proteins.

Life science laboratories apply green fluorescent proteins (GFP) to study protein localization, interaction and dynamics in fluorescence microscopy.

We develop and use technologies (robotic microscopy data acquisition, computerised 3 - D reconstruction, and digital atlasing) for efficiently assigning localization to neuroscience data.

We are developing novel techniques to achieve highest 3D resolution of single fluorophores using Supercritical Angle Localization Microscopy and correlative superresolution and electron microscopy, and are implementing quantitative superresolution imaging based on counting reference standards.

Prior to Joining IDRI, Matthew worked at the Institut de Biologie Integrative de la Cellule, France with Dr. Frederic Boccard applying super resolution microscopy to study the temporal and spatial localization of proteins involved in bacterial chromosome organization.

Correlative light and electron microscopy (CLEM) is an imaging technique that combines electron microscopy (EM) with fluorescence localization data.

Our results are the most accurate subcellular localization classifications to date, and demonstrate the usefulness of deep learning for high throughput microscopy.

Serial sections were evaluated with light microscopy (esterase reaction) and fluorescent microscopy (red fluorescence) and localization of stainings compared.