Recently, the combination
of these two techniques has produced a new generation
of experimental setups enabling the simultaneous manipulation
of a biological substrate (for example an
actin filament or a DNA molecule) and detection /
localization of an interacting partner enzyme (for example myosin or a DNA - binding protein).
Analysis
of cellular
localization of known
actin cross-linking proteins in mouse melanoma B16F1 cells revealed that fascin was specifically localized along the entire length
of all filopodia, whereas other
actin cross-linkers were not.