Moreover, annexin V / propidium iodide staining showed that VPA only marginally decreased the
viability of in vitro — differentiated Th1 and Th17
cells at a relatively high concentration (1 mM)(Supplemental Fig. 4A, 4B), whereas VPA readily inhibited the differentiation of Th1 and Th17
cells at a
low concentration (0.2 — 0.5 mM), indicating that apoptosis induction was not the primary mechanism for Th1 and Th17
cell differentiation inhibition (28, 29).
Treatment with ActD at
low doses of 3 - 30 nM for the 293 and 293T
cells, and 3 - 100 nM for the HepG2
cells, for 24 and 48 hours dose - dependently decreased
cell viability (Fig. 8).