Sentences with word «luciferase»
Luciferase is a word used to describe an enzyme that produces light. It is often found in certain organisms like fireflies or glowworms, and scientists use it to study and understand various processes in living organisms. Full definition
S3A) were assayed for luciferase activity in the presence or absence of DDX3.
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D, IRF - 9 — deficient U2A cells were transiently transfected with IRF - 1 or empty vector, the binding of exogenous IRF - 1 to RIG - G gene promoter was tested by ChIP assay, and the induction of reporter gene pXP2 (− 310) was detected with luciferase assay.
Furthermore, we observed no induction of luciferase activity in IRF - 9 — deficient U2A cells after IFNα treatment, unless the cells were reconstituted by IRF - 9 (Fig. 1D).
In these assays, both wild - type Bach1 and FLAG - hBach1 - AP4 - 7 efficiently repressed basal levels of luciferase expression.
His team looked at an experimental drug called PTC124, which the firefly luciferase assay had identified as a promising candidate for treating stop - sign forms of cystic fibrosis and which is now being tested in clinical trials.
Once they'd found suitable pairs, they constructed three components: GBPa - VP16 (activation domain); GBPb - GAL4 (DNA - binding domain); and a UAS - driven luciferase reporter construct.
The researchers engineered a strain in which a Synechococcus gene normally turned on during photosynthesis signals its activity by switching on the gene for luciferase, an enzyme from another bacterium that drives a light - emitting chemical reaction.
This application note demonstrates the first use of BRET for monitoring ligand binding to GPCRs in live cells enabled by NanoLuc ® luciferase.
Alternatively, they can stimulate luminescence in compounds such as luciferase, the chemical that makes fireflies glow.
She crystallized the enzyme luciferase and teased apart the reactions it catalyzes to generate the phosphorescent molecule responsible for the insects» glow.
AND THERE WAS LIGHT Most deep - sea anglerfish lures glow with light that is generated by an enzyme called luciferase.
All three compounds were able to induce luciferase expression, demonstrating their ability to overcome Bach1 repression of HMOX E2 - dependent transcription (Figure 6C).
They combined the conditional K - ras luciferase mice with mice conditionally inactivated for the p53 tumor suppressor, AMPK, or the LKB1 / STK11 tumor suppressor.
When G - CSF activates its receptor, it ultimately activates proteins called STATs; the team engineered the cells to churn out the light - generating protein luciferase, which would be turned on by the STAT molecules.
Nonetheless, CDDO - Me and HPP - 1014 still were able to activate HMOX1 E2 - dependent luciferase expression in the presence of mutant Bach1 proteins, indicating that the CP motifs in Bach1 are not critical for efficient derepression of Bach1 by an electrophile (Figure 6D).
The secret to the new method comes in the many tweaks and improvements that Li added to the system, which is symbolized by the acronym he and Wahl apply to the method — ReBiL — which indicates «recombinase enhanced bi-molecular luciferase complementation.»
Proteins that give off light include chemiluminescent proteins, like luciferases, as well as fluorescent ones, like Green Fluorescent Protein (GFP).
In all cases, the relative luciferase activity shown is presented as fold activation relative to the control transfection and is derived from at least three independent experiments done in triplicate.
The transfected and compound - treated cells were then gently washed with PBS followed by the addition of Luciferase substrate (Steadyliteplus, PerkinElmer).
Researchers often use bioluminescence to overcome these issues as traditional luciferases emit at near optimal wavelengths (Fluc emits at 610 nm) and do not require external light sources.
The pGL3 luciferase reporter plasmids with the wild - type or mutated 3 ′ UTR of APC were transiently transfected into HEK293T cells along with 25 nM miR - 142 precursor or negative control precursor.
Individually, these two could repair less than 2 % of clumped luciferase, but teamed with Hsp104 they brought almost half of the denatured proteins back to life.
When ATP comes into contact with the liquid - stable luciferase / luciferin reagent in the SystemSure's testing swap, light is emitted in direct proportion to the amount of ATP present.
Thus the oxyluciferin - luciferase complex is the lightemitter.
The scientists adapted an old method in which luciferase is split in half to make two non-functional fragments.
To screen for such drugs, scientists attach firefly luciferase DNA to the end of the cystic fibrosis gene.
Ten nanograms of reporter plasmid and 200 ng of miR7 expression plasmid were cotransfected with 1 ng of phRG - TK Renilla luciferase internal control plasmid (Promega) into 293TN cells using Lipofectamine 2000 reagent (Invitrogen).
This design enabled intramolecular bioluminescence resonance energy transfer (BRET) between luciferase and the NIR dye leading to generation of NIR light only in HIF - active hypoxic cells (Figure a).
Type II parasites used in preliminary studies were either Prugniaud expressing tandem dimeric tomato red fluorescent protein and OVA peptide [36](dose: 2500 parasites) or ME49 expressing luciferase [37](dose: 300 parasites).
His lab crossed the K - ras model to a ubiquitous promoter driving conditional luciferase expression only in cells that Cre has entered.
The donor luciferase enzyme can be expressed on the N - terminus of the receptor of interest in an appropriate transfected cell line.
Oct. 27, 2016 — A team of Vanderbilt scientists have genetically modified luciferase, the enzyme that produces bioluminescence, so that it acts as an optical sensor that records activity in brain cells.
The FRE - luciferase transgenic line [34] is a gift from Dr. Alexander W. Shingleton (Lake Forest College, IL).