Sentences with phrase «luciferase expression construct»

Cells were transfected with 320 ng pGL3 luciferase expression construct containing the 3 ′ UTR of human APC, 40 ng pGL4.74 hRLuc / TK Renilla luciferase vector (Promega, Fitchburg, WI), and 25 nM hsa - miR - 142 precursor or negative control precursor (Ambion).

Not exact matches

Cells were transfected with the human ID1 promoter -LRB--- 985 / +94) luciferase reporter construct (31) together with the wild - type or mutant ACVR1 expression construct.

To examine whether DDX3 transactivates the p21waf1 / cip1 promoter activity through Sp1 site - interacting proteins, the Sp1 or Sp3 expression plasmid together with the DDX3 expression construct and the p21waf1 / cip1 promoter — driven luciferase reporter were introduced into HuH - 7 cells.

p21waf1 / cip1 promoter — driven luciferase reporter (p21 - Luc; 0.05 - 0.5 μg) and increasing amount (0.1 - 2 μg) of FLAG - DDX3 expression construct were cotransfected into various cell lines as indicated.

To this end, increasing amounts of expression constructs of DDX3 / DQAD, DDX3 / AAA and wild - type DDX3 were cotransfected with p21waf1 / cip1 promoter — driven luciferase reporter.

The cells were cotransfected with the reporter gene constructs and related expression plasmids as indicated by using SuperFect (Qiagen) followed by transcriptional activity assay using Dual - Luciferase Assay System (Promega) according to the manufacturer's instruction.