When compared to unfractionated hu - PBMCs, the CD8 + - depleted hu - PBMC recipients show similar reductions in
luciferase expression following IL - 15 superagonist treatment.
The cells were cotransfected with the reporter gene constructs and related
expression plasmids as indicated by using SuperFect (Qiagen)
followed by transcriptional activity assay using Dual -
Luciferase Assay System (Promega) according to the manufacturer's instruction.