Sentences with phrase «luciferase expression in»

In contrast, the ability of both CoPP and HPP - 4382 to induce HMOX1 E2 ARE - dependent luciferase expression in the presence of the mutant Bach1 protein was sharply inhibited.

Nonetheless, CDDO - Me and HPP - 1014 still were able to activate HMOX1 E2 - dependent luciferase expression in the presence of mutant Bach1 proteins, indicating that the CP motifs in Bach1 are not critical for efficient derepression of Bach1 by an electrophile (Figure 6D).

Not exact matches

His lab crossed the K - ras model to a ubiquitous promoter driving conditional luciferase expression only in cells that Cre has entered.

As shown in Fig. 2A, exogenous expression of DDX3 in various cell lines led to a 2 - to 4-fold up - regulation of p21waf1 / cip1 promoter — driven luciferase activity.

Expression of HMOX1 E2 - dependent luciferase expression was determined in HepG2 cells co-transfected with an HMOX1 E2 - dependent reporter plasmid and a plasmid expressing FLAG - tagged wildtype Bach1 (FExpression of HMOX1 E2 - dependent luciferase expression was determined in HepG2 cells co-transfected with an HMOX1 E2 - dependent reporter plasmid and a plasmid expressing FLAG - tagged wildtype Bach1 (Fexpression was determined in HepG2 cells co-transfected with an HMOX1 E2 - dependent reporter plasmid and a plasmid expressing FLAG - tagged wildtype Bach1 (Figure 6A).

Luciferase expression was markedly lower in cells expressing Bach1, indicating effective repression of HMOX1 E2 - dependent transcription by Bach1 (Figure 6B).

In these assays, both wild - type Bach1 and FLAG - hBach1 - AP4 - 7 efficiently repressed basal levels of luciferase expression.

Second, in IRF - 9 — deficient U2A cells, IFNα - induced luciferase activity was detected only when enforcing IRF - 9 expression in these cells (Fig. 1D).

In agreement with this, both wt STAT2 and mutant STAT2 - Y690F displayed a similar effect on the luciferase reporter gene expression in U3A cells without IFNIn agreement with this, both wt STAT2 and mutant STAT2 - Y690F displayed a similar effect on the luciferase reporter gene expression in U3A cells without IFNin U3A cells without IFNα.

When compared to unfractionated hu - PBMCs, the CD8 + - depleted hu - PBMC recipients show similar reductions in luciferase expression following IL - 15 superagonist treatment.

Gene expression Reporter genes, such as luciferase or GFP, can also be assessed in microplate readers, enabling in vitro and in vivo determination of gene expression for studies using markers of genetic alteration.

(11) To more directly test for defects in Wnt signaling, CAST / EiJ and C57BL / 6J WT and mTERT — / — embryonic fibroblasts were transfected with a luciferase reporter plasmid with a Wnt3a ligand; luciferase expression was indistinguishable between WT and mTERT — / — cells.