Sentences with phrase «luciferase reporter gene»

The minor allele of rs25532 significantly decreased luciferase reporter gene expression levels by 15 — 80 %, depending on 5 - HTTLPR allele background and cell type.

C, schematic representation of the wt or mutant RIG - G promoter - luciferase reporter gene constructs.

In agreement with this, both wt STAT2 and mutant STAT2 - Y690F displayed a similar effect on the luciferase reporter gene expression in U3A cells without IFNα.

Several IFN signaling pathway — related key regulators, including STAT1, STAT2, IRF - 1, and IRF - 9, were transfected individually or in combination into STAT1 - deficient U3A cells, together with a luciferase reporter gene containing RIG - G promoter.

Neural stem cells transfected with a luciferase reporter gene construction were treated with 12 000 compounds.

Not exact matches

The cells were cotransfected with the reporter gene constructs and related expression plasmids as indicated by using SuperFect (Qiagen) followed by transcriptional activity assay using Dual - Luciferase Assay System (Promega) according to the manufacturer's instruction.

Luciferase activity was shown relative to the values obtained in the cells transfected with the same reporter gene construct and empty vectors.

This increase was accompanied by enhanced transactivation of Oct4 luciferase reporters (Figure 4C) and increased chromatin occupancy by Oct4 at native PORE (fgf12, p < 0.01) and non-PORE (Nanog, which is regulated by an Oct4 / Sox2 heterodimer, p < 0.005) target genes (Figure 3D).

Gene expression Reporter genes, such as luciferase or GFP, can also be assessed in microplate readers, enabling in vitro and in vivo determination of gene expression for studies using markers of genetic alteratGene expression Reporter genes, such as luciferase or GFP, can also be assessed in microplate readers, enabling in vitro and in vivo determination of gene expression for studies using markers of genetic alteratgene expression for studies using markers of genetic alteration.