Sentences with phrase «luciferase reporter plasmids»

The pGL3 luciferase reporter plasmids with the wild - type or mutated 3 ′ UTR of APC were transiently transfected into HEK293T cells along with 25 nM miR - 142 precursor or negative control precursor.

D, KLF4 3 ′ - UTR luciferase reporter plasmid was transfected in 293TN cells with indicated miRNAs or LNA.

For DNA transfection, the HepG2, 293, and 293T cells were seeded respectively at 2 × 105, 2 × 105, and 1 × 105 cells / well in 24 - well plates overnight, and then the p53 - TA - Luc luciferase reporter plasmid and RSV - lacZ plasmids were transiently transfected into the cells using NTRII for 6 h, followed by treatment with the test compounds.

(11) To more directly test for defects in Wnt signaling, CAST / EiJ and C57BL / 6J WT and mTERT — / — embryonic fibroblasts were transfected with a luciferase reporter plasmid with a Wnt3a ligand; luciferase expression was indistinguishable between WT and mTERT — / — cells.

Not exact matches

Ten nanograms of reporter plasmid and 200 ng of miR7 expression plasmid were cotransfected with 1 ng of phRG - TK Renilla luciferase internal control plasmid (Promega) into 293TN cells using Lipofectamine 2000 reagent (Invitrogen).

To examine whether DDX3 transactivates the p21waf1 / cip1 promoter activity through Sp1 site - interacting proteins, the Sp1 or Sp3 expression plasmid together with the DDX3 expression construct and the p21waf1 / cip1 promoter — driven luciferase reporter were introduced into HuH - 7 cells.

Expression of HMOX1 E2 - dependent luciferase expression was determined in HepG2 cells co-transfected with an HMOX1 E2 - dependent reporter plasmid and a plasmid expressing FLAG - tagged wildtype Bach1 (Figure 6A).

The cells were cotransfected with the reporter gene constructs and related expression plasmids as indicated by using SuperFect (Qiagen) followed by transcriptional activity assay using Dual - Luciferase Assay System (Promega) according to the manufacturer's instruction.