Sentences with phrase «luciferase reporters containing»

Not exact matches

The p21waf1 / cip1 promoter — driven luciferase reporters (pGL3 derivatives) containing point mutations in six Sp1 elements, (Mut1) p21 - Luc, (Mut2) p21 - Luc, (Mut3) p21 - Luc, (Mut4) p21 - Luc, and (Mut5 / 6) p21 - Luc and the parental wild - type reporter, (Wt) p21 - Luc, were kindly provided by Dr. D. Kardassis (Department of Basic Sciences, University of Crete Medical School, Heraklion, Crete, Greece; ref.

A, schematic representation of serial p21waf1 / cip1 promoter — driven luciferase reporters used in transactivation assay: p21 - Luc contains the 2.3 - kb full - length of p21waf1 / cip1 promoter (from − 2,326 to +10 nucleotide related transcription start site); its derivatives (− 159 / +8) p21 - Luc, (− 123 / +8) p21 - Luc, (− 84 / +8) p21 - Luc, (− 76 / +8) p21 - Luc, (− 63 / +8) p21 - Luc, and (− 56 / +8) p21 - Luc contain a series of deleted promoters of p21waf1 / cip1.

Several IFN signaling pathway — related key regulators, including STAT1, STAT2, IRF - 1, and IRF - 9, were transfected individually or in combination into STAT1 - deficient U3A cells, together with a luciferase reporter gene containing RIG - G promoter.