Sentences with phrase «luciferase reporters used»
A, schematic representation of serial p21waf1 / cip1 promoter — driven luciferase reporters used in transactivation assay: p21 - Luc contains the 2.3 - kb full - length of p21waf1 / cip1 promoter (from − 2,326 to +10 nucleotide related transcription start site); its derivatives (− 159 / +8) p21 - Luc, (− 123 / +8) p21 - Luc, (− 84 / +8) p21 - Luc, (− 76 / +8) p21 - Luc, (− 63 / +8) p21 - Luc, and (− 56 / +8) p21 - Luc contain a series of deleted promoters of p21waf1 / cip1.
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After 24 hours, luciferase activities were measured by using dual - luciferase reporter assay system (Promega).
Ten nanograms of reporter plasmid and 200 ng of miR7 expression plasmid were cotransfected with 1 ng of phRG - TK Renilla luciferase internal control plasmid (Promega) into 293TN cells using Lipofectamine 2000 reagent (Invitrogen).
To more fully explore the effects of compound on Bach1 activity, a luciferase reporter assay using the HMOX1 E2 ARE as a target was developed.
The clone, pGL - MARE - Luc, was confirmed via DNA sequencing before being used in the luciferase reporter assay.
Luciferase activities were measured using the Dual - Luciferase Reporter Assay kit (Promega) following the manufacturer's instructions.
The cells were cotransfected with the reporter gene constructs and related expression plasmids as indicated by using SuperFect (Qiagen) followed by transcriptional activity assay using Dual - Luciferase Assay System (Promega) according to the manufacturer's instruction.
The Dual - Luciferase Reporter Assay System (E1910; Promega, Madison, WI) was used to perform dual - reporter assays on NFκB luciferase and Renilla luciferase (internalLuciferase Reporter Assay System (E1910; Promega, Madison, WI) was used to perform dual - reporter assays on NFκB luciferase and Renilla luciferase (internal cReporter Assay System (E1910; Promega, Madison, WI) was used to perform dual - reporter assays on NFκB luciferase and Renilla luciferase (internal creporter assays on NFκB luciferase and Renilla luciferase (internalluciferase and Renilla luciferase (internalluciferase (internal control).
For DNA transfection, the HepG2, 293, and 293T cells were seeded respectively at 2 × 105, 2 × 105, and 1 × 105 cells / well in 24 - well plates overnight, and then the p53 - TA - Luc luciferase reporter plasmid and RSV - lacZ plasmids were transiently transfected into the cells using NTRII for 6 h, followed by treatment with the test compounds.
The researchers took a FRET reporter and adapted it into a BRET reporter by using NanoLuc ® Luciferase to excite the Venus fluorescent protein in conjunction with a membrane - inserted voltage sensing domain (LOTUS V; Luminescent Optical Tool for Universal Sensing of Voltage).
Gene expression Reporter genes, such as luciferase or GFP, can also be assessed in microplate readers, enabling in vitro and in vivo determination of gene expression for studies using markers of genetic alteration.
Luciferase activities were measured using the Dual - Luciferase Reporter Assay System (Promega) and normalized to Renilla luciferaseLuciferase activities were measured using the Dual - Luciferase Reporter Assay System (Promega) and normalized to Renilla luciferaseLuciferase Reporter Assay System (Promega) and normalized to Renilla luciferaseluciferase activity.