To validate their theory, the researchers compared their results with contact maps of
lymphoblast cells generated by Hi - C.
Both stages of the antibody response — the induction of helper
T lymphoblasts by dendritic cells and the delivery of help from T to B cell — occur in discrete cell aggregates that can be isolated by velocity sedimentation.
Co-lead authors and Rice postdoctoral fellows Michele Di Pierro and Ryan Cheng used ChIP - sequencing data for a
human lymphoblast cell that probes 84 different DNA - binding proteins and 11 chemical modifications of histones.
The researchers found higher levels of NCL - like lipofuscin
in lymphoblasts, cells that mature into lymphocytes in patients with NCL, and also in asymptomatic patients carrying mutations in the progranulin gene.
To study the effectiveness of sulforaphane, the scientists incubated human - derived leukemic cell lines and
primary lymphoblasts from pediatric patients with the compound.
There is an increase in nondegenerate neutrophilsm, small to intermediate mature lymphocytes, rare macrophages, rare plasma cells, and
rare lymphoblasts seen intimately associated with hepatocellular clusters.
When
the lymphoblasts develop suddenly, the condition is called acute lymphoblastic leukemia (ALL).