Sentences with phrase «lysate after»
Anti-HIF-1-alpha unpurified antibody (ab51608) reactivity with reduced Hep3B cell lysate after transient transfection of scrambled siRNA (lanes1 - 3 and 7 - 9) or HIF -1-alpha siRNA (lanes 4 - 6 and 10 - 12).
http://www.abcam.com/ Not exact matches
But after a while, I realized that I understood the rabbit reticulocyte lysate system much better than anybody else in the entire world, because I had made every single mistake that was possible to make and learned from them.
Whole - cell lysates were prepared from control and infected cells 48 hours after infection.
After 36 h, cell lysates were analyzed by Western blot as indicated.
Sera from mice obtained 3 weeks after tick - feeding were tested against recombinant B. burgdorferi antigens and whole - cell B. burgdorferi lysates.
Twelve hours after transfection, cells were treated with or without IFNα for another 24 h. Cell lysates (Input) were then immunoprecipitated (IP) with anti — IRF - 9 antibody followed by Western blot (WB) analysis with anti-STAT2 antibody.
Lysates were harvested 3 days after transfection and subjected to immunoblotting.