Sentences with phrase «lysates from»

Lysates from human embryonal kidney (HEK) 293T cells transfected with or without the precursor for miR - 142 or miR - 150 were immunopurified using an anti-Ago antibody.

Whole - cell lysates from B. burgdorferi and from Escherichia coli expressing recombinant P39 [BmpA (Borrelia membrane protein A)-RSB-(19) or recombinant His - tagged OspC (20) were prepared as described in ref.

Western blot analysis of antibody specificity has been done using a routine sample setup composed of IgG / HSA - depleted human plasma and protein lysates from a limited number of human tissues and cell lines.

Notably, analysis of cell lysates from ABL2 - depleted and ABL1 / ABL2 double - knockdown breast cancer cells showed a greater reduction of the phosphorylation of CrkL compared to cells with knockdown of ABL1 alone (Fig. 2K).

(I) Immunoblotting was performed using the indicated antibodies on whole - cell lysates from cells incubated or not with TRAIL.

Lysates from these two cell populations were fractionated on the basis of the subcellular localization using the ProteoExtract Kit, and 40 μg of proteins was loaded for Western blotting.

(Top) An in vitro kinase assay was carried out by IP overnight with 1 μg of the rabbit anti-RIPK2 antibody from ProteinTech (Rosemont, IL) and 1 ml of lysate from a confluent six - well dish of KMH2 cells.

Not exact matches

Larger biomarker signatures can be detected with technology from CDI Laboratories, which offers microarrays of functional human proteins (over 20,000 on a single array) to test the antibodies present in human liquid biopsy samples, such as blood, serum, plasma, CSF, or tissue lysates.

But after a while, I realized that I understood the rabbit reticulocyte lysate system much better than anybody else in the entire world, because I had made every single mistake that was possible to make and learned from them.

Each lysate product is sold in kit format consisting of a KO cell lysate and a parental cell lysate, which are immediately available from OriGene for the academic and industrial research markets.

Derived mostly from human embryonic kidney 293T (HEK293T) and HeLa cell lines, EdiGene Knockout (KO) Cell Lysates have been optimized through the use of genome editing technology and validated at the genomic level through PCR and Sanger - sequencing techniques to ensure the accuracy and knockout of the target gene.

The research, performed using recombinant protein from cell lysate isolated in vitro, also validates a methodology for characterizing large, multivalent membrane proteins in general.

Target detectability data from 858 samples from 11 independent customer sites, utilizing five unique sample types: serum, plasma, cell culture supernatant, lysates, and bronchoalveolar lavage.

Normalized protein lysates were subjected to SDS - PAGE, transferred to nitrocellulose, and immunoblotted with primary Abs from Cell Signaling Technology (Danvers, MA) at concentrations recommended by the manufacturer.

Unpurified ab32518 used to immunoprecipitate IKB alpha from human HeLa whole cell lysate.

Unpurified ab32518 used to immunoprecipitate IKB alpha from rat PC12 whole cell lysate.

Aβ 1 to 40 and 1 to 42 levels in hippocampal lysate were analyzed using the Aβ1 - 40 and Aβ1 - 42 ELISA kits from Invitrogen (# KHB3481 and # KHB3442, respectively).

Cell lysates of hippocampal tissue from the aged huAPP / PS1 and control mice were analyzed for an effect of J147 on the APP processing pathway by immunoblotting with antibodies against BACE (D) and APP (E).

Cell lysates of either hippocampal tissue or entorhinal cortex tissue from aged AD mice on control diet (AD Ctl) or J147 diet (AD J147) were analyzed by Western blotting and the images quantified in bar graphs accompanying the images.

Whole - cell lysates were prepared from control and infected cells 48 hours after infection.

Immunoblot analysis of recombinant antigens and B. burgdorferi lysates with representative sera from mice inoculated by i.p. / s.c. routes with B31 - A3 WT, ospC7 mutant, and ospC7 / ospC +4 complemented B. burgdorferi clones.

Normal translation of human adenovirus mRNA in cell - free lysates prepared from abortively as well as productively infected monkey cells

Sera from mice obtained 3 weeks after tick - feeding were tested against recombinant B. burgdorferi antigens and whole - cell B. burgdorferi lysates.

Unmatched performance — high transfection efficiency & cell viability in primary cells Fully scalable — from R&D to the clinic without reoptimization Safe — non-viral cell engineering in closed, sterile, computer - controlled environment Cell loading flexibility — mRNA, gRNA, siRNA, DNA, proteins, cell lysates, and small molecules into autologous or allogenic immune, stem / progenitor, or somatic cells Regulatory ease — Master File designation with the CBER Division of the U.S. FDA, cleared by NIH's RAC and Health Canada, cGMP - compliant, and CE - marked

Gilbertie also recently received a two - year, $ 100,000 fellowship grant from the Morris Animal Foundation for her platelet lysate therapy research.