Sentences with phrase «lysates of»

... «Preliminary experimental and clinical data indicated that the cell lysates of L. rhamnosus V are highly effective in prevention and treatment of infectious diseases, common allergies (food allergy, bronchitis, hay fever, and asthma), hepatitis C, chronic fatigue, and fibromyalgia.»

Surprisingly, we did not observe any increase in VCAM1 in either the TBS fraction or total cell lysates of brain homogenates (Fig 6C).

Lysates of primary human tumors were subjected to immunoblotting using the indicated antibodies.

The Assay Designs ® SMN (human) Enzyme Immunometric Assay (EIA) kit is a complete kit for the quantitative determination of SMN protein in cell lysates of human origin.

(A to C) Immunoblots with the indicated antibodies were performed on whole - cell lysates of 1833 and SCP28 cells.

Cell lysates of either hippocampal tissue or entorhinal cortex tissue from aged AD mice on control diet (AD Ctl) or J147 diet (AD J147) were analyzed by Western blotting and the images quantified in bar graphs accompanying the images.

Cell lysates of hippocampal tissue from the aged huAPP / PS1 and control mice were analyzed for an effect of J147 on the APP processing pathway by immunoblotting with antibodies against BACE (D) and APP (E).

Total cell lysates of HuH - 7 cells were incubated with GST or GST - Sp1 fusion protein — prebound glutathione - Sepharose 4B beads and the bound fractions were subjected to immunoblotting with antibody against DDX3 (top).

As seen in in vitro co-culture studies, EphA2, EphB2 and EphB4 phosphorylation was reduced in whole tumour lysates of Tie2PEKO animals, independently of changes in gene expression (Supplementary Fig. 10).

Lysate of cross-linked HEK293T cells was immunoprecipitated by an anti-β-catenin antibody or mouse IgG.

Not exact matches

Larger biomarker signatures can be detected with technology from CDI Laboratories, which offers microarrays of functional human proteins (over 20,000 on a single array) to test the antibodies present in human liquid biopsy samples, such as blood, serum, plasma, CSF, or tissue lysates.

Each lysate product is sold in kit format consisting of a KO cell lysate and a parental cell lysate, which are immediately available from OriGene for the academic and industrial research markets.

Derived mostly from human embryonic kidney 293T (HEK293T) and HeLa cell lines, EdiGene Knockout (KO) Cell Lysates have been optimized through the use of genome editing technology and validated at the genomic level through PCR and Sanger - sequencing techniques to ensure the accuracy and knockout of the target gene.

William B. Coley creates a filtered mixture of bacteria and bacterial lysates, composed of Streptococcus pyogenes and Bacillus prodigiosus, called «Coley's Toxins,» to treat tumors.

Lysates were immunoblotted for protein levels of (A) phosphorylated ERK (pERK), total ERK (tERK), and β - actin or (B) phosphorylated IκBα (p - IκBα), total IκBα (IκBα), and β - actin.

Rat brain tissue lysate was subjected to SDS PAGE followed by western blot with 23449 -1-AP (UBQLN2 antibody) at a dilution of 1:800 incubated at room temperature for 1.5 hours.

The Clinical Biomarkers Facility offers services for high - throughput and highly specific analyses of protein biomarker candidates in body fluids such as plasma, serum, cerebrospinal fluids etc. and cell and tissue lysates using molecular tools such as proximity extension and proximity ligation technologies (PEA and PLA) providing assays with high specificity and sensitivity in complex biological matrices.

Co-culture of Tie2 - silenced pericytes with EC resulted in reduced EphA2, EphB2 and EphB4 phosphorylation in the co-culture lysates (Fig. 2h).

Subsequently, lungs were digested with DMEM containing 1.25 mM CaCl2, 200 U ml − 1 collagenase I and 10 μg ml − 1 DNase I at 37 °C for 1 h. Single - cell suspensions of the digested organ were prepared by passing through 18G and 19G cannula syringes and filtering the lysates through a 100 μm cell strainer.

Lysates from these two cell populations were fractionated on the basis of the subcellular localization using the ProteoExtract Kit, and 40 μg of proteins was loaded for Western blotting.

The concentration of protease inhibitor should be 1.5 — 2 times that of a typical lysate preparation protocol for Western blotting.

Live cell assays constructed with genetically encoded, fluorescent biosensors can provide significant advantages over endpoint assays measured in cell lysates because functional information about the timing and location of cellular responses can be monitored in cells that are relevant to disease.

(E) Immunoblots were performed on whole - cell lysates (pSTAT5, STAT5, and tubulin) or conditioned medium (MMP1, IL - 6, and TNC) of parental 1833 and SCP28 cells.

All genes with Data reliability «Supported» in one or both of the two methods immunohistochemistry and immunofluorescence, or standard validation «Supported» for the Western blot application (assays using over-expression lysates not included) are classified as «Evidence at protein level».

Following the provided protocol, a portion of the RNA - containing lysate (5 — 10 µl) was hybridized at 54 degrees C overnight to RNA specific magnetic capture beads in the presence of blocking buffers, proteinase K and preordered mRNA probe sets specific for the genes of interest: HMOX1, Nrf2, Keap1, Bach1, and GAPDH.

One - half volume of 3x Sample buffer [6.7 % SDS, 160 mM Tris - HCl pH 6.8,.005 % Bromophenol Blue dye, 8.3 % glycerol, 15 % 2 - BME) was added to the lysates.

Notably, analysis of cell lysates from ABL2 - depleted and ABL1 / ABL2 double - knockdown breast cancer cells showed a greater reduction of the phosphorylation of CrkL compared to cells with knockdown of ABL1 alone (Fig. 2K).

Western blot analysis of antibody specificity has been done using a routine sample setup composed of IgG / HSA - depleted human plasma and protein lysates from a limited number of human tissues and cell lines.

Immunoblot analysis of recombinant antigens and B. burgdorferi lysates with representative sera from mice inoculated by i.p. / s.c. routes with B31 - A3 WT, ospC7 mutant, and ospC7 / ospC +4 complemented B. burgdorferi clones.

Cell lysates were directly subjected to Western blot to assess the expression of STAT6 and p300.

Western blot analysis of whole - cell lysates demonstrated that ospC7 no longer synthesized OspC (Fig. 3).

The PGE2 concentration was corrected by the protein concentration of the cell lysates and presented as pg / μg protein.

At the end of the desired treatment times, cell lysates were prepared in lysis buffer (1 % NP - 40, 0.5 mM Tris - HCl (pH 7.5), 0.14 M NaCl, 5 mM KCl, 5 mM EDTA, and 1 mM phenylmethylsulfonyl fluoride) plus serine / threonine phosphatase inhibitor cocktail.

Antibody titers of positive control anti-sera and reactivity of pre-immune sera to polytropic MuLV - infected (upper panel) and uninfected (lower panel) HeLa cell crude cell lysates in WB testing.

Normal translation of human adenovirus mRNA in cell - free lysates prepared from abortively as well as productively infected monkey cells

We thank D. Dorward and O. Steele - Mortimer for help with confocal microscopy; S. Raffel and G. Sylva for sequencing; T. Hackstadt for assistance with photography; M. Schrumpf for supplying protein lysates and E. coli strains; R. Gilmore, M. L. Mbow, and O. Steele - Mortimer for providing antibodies; K. Caughey, G. Hettrick, and A. Mora for graphic support; and F. DeLeo, T. Hackstadt, and S. Priola for critical review of the manuscript.

(Top) An in vitro kinase assay was carried out by IP overnight with 1 μg of the rabbit anti-RIPK2 antibody from ProteinTech (Rosemont, IL) and 1 ml of lysate from a confluent six - well dish of KMH2 cells.

Protein concentrations of the lysates were determined using the BioRad DC Protein Assay (Hercules, CA).

Unmatched performance — high transfection efficiency & cell viability in primary cells Fully scalable — from R&D to the clinic without reoptimization Safe — non-viral cell engineering in closed, sterile, computer - controlled environment Cell loading flexibility — mRNA, gRNA, siRNA, DNA, proteins, cell lysates, and small molecules into autologous or allogenic immune, stem / progenitor, or somatic cells Regulatory ease — Master File designation with the CBER Division of the U.S. FDA, cleared by NIH's RAC and Health Canada, cGMP - compliant, and CE - marked

5 µL of each lysate was transferred to a white 384 well plate (Corning) and assayed by addition of 25 µL Luciferase Assay Reagent (LAR, Promega) and 25 µL Stop & Glo Reagent (Promega).

Finally, total radioactivity and total cellular protein of the lysate were determined.

Anti-HIF-1-alpha unpurified antibody (ab51608) reactivity with reduced Hep3B cell lysate after transient transfection of scrambled siRNA (lanes1 - 3 and 7 - 9) or HIF -1-alpha siRNA (lanes 4 - 6 and 10 - 12).

This unique blend of probiotic lysate and grapeseed extract works with your skin's natural process to restore and prolong your oh - so - glowing appearance.

METHODS: We treated 3T3 - L1 adipocytes with 2.5 mmol / l R (+) alpha - lipoic acid for 2 to 60 min, followed by assays of: 2 - deoxyglucose uptake; glucose transporter 1 and 4 (GLUT1 and GLUT4) subcellular localization; tyrosine phosphorylation of the insulin receptor or of the insulin receptor substrate - 1 in cell lysates; association of phosphatidylinositol 3 - kinase activity with immunoprecipitates of proteins containing phosphotyrosine or of insulin receptor substrate - 1 using a in vitro kinase assay; association of the p85 subunit of phosphatidylinositol 3 - kinase with phosphotyrosine proteins or with insulin receptor substrate - 1; and in vitro activity of immunoprecipitated Akt1.

That means — beside boosting immunity with lysates and things that have worked for you in the past — taking Vitamin C in bowel tolerance doses 2 - 3 times and megadoses (10 - 30.000 IU) of Vitamin D with cofactor vitamin K (full spectrum) so you can't overdose it because of the cofactor, every single day, for the rest of your life, to boost your immunity.

As you mention the microbiome yourself, I am wondering if you have researched probiotics, soil based organisms or probiotic lysates for allergies, as they seem to be one of the keys to eliminate them, either that their introduction replaces the bad guys in our guts or because it educates are gut cells how to properly behave to food allergens or also by eliminating / digesting them.

http://scirp.org/journal/PaperInformation.aspx?paperID=46547 • Oral administration of bacterial lysates attenuates experimental food allergy http://ncbi.nlm.nih.gov/pubmed/21597300 • Health Benefits of Organic vs. Conventional Milk «Loaded with healthy bacteria that are good for your gastrointestinal tract.»

Gilbertie, cited for her study of platelet lysate therapy for infectious arthritis, is part of the Comparative Biomedical Sciences Graduate Program.