Sentences with phrase «lysates overnight»
Not exact matches
Cell lysates were incubated with protein G sepharose beads (GE Healthcare) and 3 μg Tie2 antibody (Millipore, clone Ab33, # 05 - 584) overnight at 4 °C on a rotator.
http://www.nature.com/
Following the provided protocol, a portion of the RNA - containing lysate (5 — 10 µl) was hybridized at 54 degrees C overnight to RNA specific magnetic capture beads in the presence of blocking buffers, proteinase K and preordered mRNA probe sets specific for the genes of interest: HMOX1, Nrf2, Keap1, Bach1, and GAPDH.
(Top) An in vitro kinase assay was carried out by IP overnight with 1 μg of the rabbit anti-RIPK2 antibody from ProteinTech (Rosemont, IL) and 1 ml of lysate from a confluent six - well dish of KMH2 cells.