Sentences with phrase «lysates prepared»

Normal translation of human adenovirus mRNA in cell - free lysates prepared from abortively as well as productively infected monkey cells

Not exact matches

Subsequently, lungs were digested with DMEM containing 1.25 mM CaCl2, 200 U ml − 1 collagenase I and 10 μg ml − 1 DNase I at 37 °C for 1 h. Single - cell suspensions of the digested organ were prepared by passing through 18G and 19G cannula syringes and filtering the lysates through a 100 μm cell strainer.

Whole - cell lysates were prepared 24 and 48 hours following treatment.

Whole - cell lysates were prepared from control and infected cells 48 hours after infection.

For the coimmunoprecipitation experiments, HuH - 7 cells transfected with pcDNA - SRα / FLAG - Sp 1 were harvested and cell lysates were prepared using immunoprecipitation lysis buffer [20 mmol / L Tris - Cl (pH 7.5), 150 mmol / L NaCl, 10 % glycerol, and 1 % Triton X-100].

Karpas 299 and SU - DHL1 cells were transiently transfected with 20 μg mTOR siRNA and whole - cell lysates were prepared at 48 hours.

At the end of the desired treatment times, cell lysates were prepared in lysis buffer (1 % NP - 40, 0.5 mM Tris - HCl (pH 7.5), 0.14 M NaCl, 5 mM KCl, 5 mM EDTA, and 1 mM phenylmethylsulfonyl fluoride) plus serine / threonine phosphatase inhibitor cocktail.

Whole - cell lysates from B. burgdorferi and from Escherichia coli expressing recombinant P39 [BmpA (Borrelia membrane protein A)-RSB-(19) or recombinant His - tagged OspC (20) were prepared as described in ref.