Sentences with phrase «lysates with»

Immunoblot analysis of recombinant antigens and B. burgdorferi lysates with representative sera from mice inoculated by i.p. / s.c. routes with B31 - A3 WT, ospC7 mutant, and ospC7 / ospC +4 complemented B. burgdorferi clones.

Not exact matches

Using the kits, you can quickly and easily label proteins in lysates or purified proteins with near - infrared CF ® dyes before electrophoresis and blotting.

Larger biomarker signatures can be detected with technology from CDI Laboratories, which offers microarrays of functional human proteins (over 20,000 on a single array) to test the antibodies present in human liquid biopsy samples, such as blood, serum, plasma, CSF, or tissue lysates.

Rat brain tissue lysate was subjected to SDS PAGE followed by western blot with 23449 -1-AP (UBQLN2 antibody) at a dilution of 1:800 incubated at room temperature for 1.5 hours.

Normalized protein lysates were subjected to SDS - PAGE, transferred to nitrocellulose, and immunoblotted with primary Abs from Cell Signaling Technology (Danvers, MA) at concentrations recommended by the manufacturer.

Cell lysates were incubated with protein G sepharose beads (GE Healthcare) and 3 μg Tie2 antibody (Millipore, clone Ab33, # 05 - 584) overnight at 4 °C on a rotator.

The Clinical Biomarkers Facility offers services for high - throughput and highly specific analyses of protein biomarker candidates in body fluids such as plasma, serum, cerebrospinal fluids etc. and cell and tissue lysates using molecular tools such as proximity extension and proximity ligation technologies (PEA and PLA) providing assays with high specificity and sensitivity in complex biological matrices.

Co-culture of Tie2 - silenced pericytes with EC resulted in reduced EphA2, EphB2 and EphB4 phosphorylation in the co-culture lysates (Fig. 2h).

Subsequently, lungs were digested with DMEM containing 1.25 mM CaCl2, 200 U ml − 1 collagenase I and 10 μg ml − 1 DNase I at 37 °C for 1 h. Single - cell suspensions of the digested organ were prepared by passing through 18G and 19G cannula syringes and filtering the lysates through a 100 μm cell strainer.

Total cell lysates of HuH - 7 cells were incubated with GST or GST - Sp1 fusion protein — prebound glutathione - Sepharose 4B beads and the bound fractions were subjected to immunoblotting with antibody against DDX3 (top).

The assay has been validated for use with human and mouse cell lysates and tissue homogenates, such as peripheral blood mononuclear cell (PBMC) lysates, and will be marketed through Enzo's worldwide sales and marketing group.

Cell lysates of hippocampal tissue from the aged huAPP / PS1 and control mice were analyzed for an effect of J147 on the APP processing pathway by immunoblotting with antibodies against BACE (D) and APP (E).

For the coimmunoprecipitation experiments, HuH - 7 cells transfected with pcDNA - SRα / FLAG - Sp 1 were harvested and cell lysates were prepared using immunoprecipitation lysis buffer [20 mmol / L Tris - Cl (pH 7.5), 150 mmol / L NaCl, 10 % glycerol, and 1 % Triton X-100].

Live cell assays constructed with genetically encoded, fluorescent biosensors can provide significant advantages over endpoint assays measured in cell lysates because functional information about the timing and location of cellular responses can be monitored in cells that are relevant to disease.

Karpas 299 and SU - DHL1 cells were transiently transfected with 20 μg mTOR siRNA and whole - cell lysates were prepared at 48 hours.

Antibodies with an uncertain routine WB have been revalidated using an over-expression lysate (VERIFY Tagged Antigen (TM), OriGene Technologies, Rockville, MD) as a positive control.

All genes with Data reliability «Supported» in one or both of the two methods immunohistochemistry and immunofluorescence, or standard validation «Supported» for the Western blot application (assays using over-expression lysates not included) are classified as «Evidence at protein level».

(I) Immunoblotting was performed using the indicated antibodies on whole - cell lysates from cells incubated or not with TRAIL.

Cell lysates were subjected to western blotting and sequentially probed with Abs to phosphorylated p38 (phospho - p38), total p38 or actin.

(A to C) Immunoblots with the indicated antibodies were performed on whole - cell lysates of 1833 and SCP28 cells.

Notably, analysis of cell lysates from ABL2 - depleted and ABL1 / ABL2 double - knockdown breast cancer cells showed a greater reduction of the phosphorylation of CrkL compared to cells with knockdown of ABL1 alone (Fig. 2K).

Precleared nuclear lysates were incubated with antibodies against Nrf2 or Bach1.

We thank D. Dorward and O. Steele - Mortimer for help with confocal microscopy; S. Raffel and G. Sylva for sequencing; T. Hackstadt for assistance with photography; M. Schrumpf for supplying protein lysates and E. coli strains; R. Gilmore, M. L. Mbow, and O. Steele - Mortimer for providing antibodies; K. Caughey, G. Hettrick, and A. Mora for graphic support; and F. DeLeo, T. Hackstadt, and S. Priola for critical review of the manuscript.

(Top) An in vitro kinase assay was carried out by IP overnight with 1 μg of the rabbit anti-RIPK2 antibody from ProteinTech (Rosemont, IL) and 1 ml of lysate from a confluent six - well dish of KMH2 cells.

Twelve hours after transfection, cells were treated with or without IFNα for another 24 h. Cell lysates (Input) were then immunoprecipitated (IP) with anti — IRF - 9 antibody followed by Western blot (WB) analysis with anti-STAT2 antibody.

In some cases, leftover lysate was spun briefly and mixed 3 ∶ 1 with LDS western blot loading buffer (Invitrogen) + β mercaptoethanol, heated at 70 °C for 10 minutes and stored at − 20 °C until use.

We validated FFId based on a public benchmark dataset, comprising a yeast cell lysate spiked with protein standards that provide a known ground - truth.

Unmatched performance — high transfection efficiency & cell viability in primary cells Fully scalable — from R&D to the clinic without reoptimization Safe — non-viral cell engineering in closed, sterile, computer - controlled environment Cell loading flexibility — mRNA, gRNA, siRNA, DNA, proteins, cell lysates, and small molecules into autologous or allogenic immune, stem / progenitor, or somatic cells Regulatory ease — Master File designation with the CBER Division of the U.S. FDA, cleared by NIH's RAC and Health Canada, cGMP - compliant, and CE - marked

The antibody was incubated under agitation with Protein G beads for 10 min, HeLa DFO treated whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10 min under agitation.

Anti-HIF-1-alpha unpurified antibody (ab51608) reactivity with reduced Hep3B cell lysate after transient transfection of scrambled siRNA (lanes1 - 3 and 7 - 9) or HIF -1-alpha siRNA (lanes 4 - 6 and 10 - 12).

Lysates were mixed with LDS loading buffer as described above, and ran on 10 % SDS - PAGE gels.

Lysates were pre-cleared with Protein A-agarose beads blocked with 2.5 mg / mL sonicated salmon sperm DNA (Sigma - Aldrich) and 0.1 % bovine serum albumin (BSA, Santa Cruz Biotechnology).

Lysates from human embryonal kidney (HEK) 293T cells transfected with or without the precursor for miR - 142 or miR - 150 were immunopurified using an anti-Ago antibody.

This unique blend of probiotic lysate and grapeseed extract works with your skin's natural process to restore and prolong your oh - so - glowing appearance.

METHODS: We treated 3T3 - L1 adipocytes with 2.5 mmol / l R (+) alpha - lipoic acid for 2 to 60 min, followed by assays of: 2 - deoxyglucose uptake; glucose transporter 1 and 4 (GLUT1 and GLUT4) subcellular localization; tyrosine phosphorylation of the insulin receptor or of the insulin receptor substrate - 1 in cell lysates; association of phosphatidylinositol 3 - kinase activity with immunoprecipitates of proteins containing phosphotyrosine or of insulin receptor substrate - 1 using a in vitro kinase assay; association of the p85 subunit of phosphatidylinositol 3 - kinase with phosphotyrosine proteins or with insulin receptor substrate - 1; and in vitro activity of immunoprecipitated Akt1.

That means — beside boosting immunity with lysates and things that have worked for you in the past — taking Vitamin C in bowel tolerance doses 2 - 3 times and megadoses (10 - 30.000 IU) of Vitamin D with cofactor vitamin K (full spectrum) so you can't overdose it because of the cofactor, every single day, for the rest of your life, to boost your immunity.

http://scirp.org/journal/PaperInformation.aspx?paperID=46547 • Oral administration of bacterial lysates attenuates experimental food allergy http://ncbi.nlm.nih.gov/pubmed/21597300 • Health Benefits of Organic vs. Conventional Milk «Loaded with healthy bacteria that are good for your gastrointestinal tract.»

The Heart for Companion Animal Research at Colorado State College has shown that cats vaccinated with FVRCP vaccines grown on Crandell - Rees Feline Kidney (CRFK) cell traces can develop antibodies to renal (kidney) proteins, and that cats hypersensitized to CRFK cell lysates can develop interstitial nephritis.