Sentences with phrase «lysed by»

In preclinical research, tumor cells lysed by coxsackievirus A21 (CVA21), a naturally occurring «common cold» ICAM - 1 — targeted RNA virus, induced a secondary systemic host - generated antitumor immune response.

Cells were washed with PBS three times and lysed by adding a modified RIPA buffer (50 mM Tris / HCl pH 7.4; 150 mM NaCl, 1 mM EDTA, 1 % Igepal, 0.25 % Na - deoxycholate).

Not exact matches

EGFR / WT and KO cells were cultured overnight and starved for 24 h. Cells were treated with capsaicin (50 μmol / L) for 30 min and then TPA (20 ng / mL) for 2 h. Cells were lysed and cox - 2 mRNA levels were analyzed by RT - PCR.

EGFR / WT and KO MEFs were treated with capsaicin (0, 10, or 50 μmol / L) for 30 min followed by TPA (20 ng / mL) for 4 h. Cells were lysed and COX - 2 expression analyzed by Western blot.

Cells were lysed and membrane and cytosolic fractions separated and EGFR abundance determined by Western blot.

The metallopolymer by itself even demonstrated antimicrobial properties, lysing bacterial cells while leaving human red blood cells unaffected.

An examination into the host's immune response to symbiont presence suggests that firebugs actively harvest their bacterial partners by lysing, or bursting open, the bacterial cells.

Cultured cells were lysed in radioimmunoprecipitation assay (RIPA) buffer (50 μl RIPA per 1 × 106 cells; 50 mM Tris / HCl; 150 mM NaCl, 1 % Triton X-100, 1 mM EDTA, 1 mM NaF, 1 mM Na3VO4, 1 μg / ml leupeptin and 1 μg / ml pepstatin) for 1 h on ice, and insoluble debris was removed by centrifugation.

After 24 h, cells were incubated with or without PP2, lysed, and immunoprecipitated with anti-HA (left) or anti-myc (right) or control Abs (ctr Ab) followed by Western blotting with the indicated Abs.

Essentially, this acts as a method of «pre-digesting» the protein by separating (i.e. lysing) peptide bonds; hence the time for digestion and absorption of amino acids will be reduced.

The four - day programme, which runs during the Offprint London art publishing fair, includes workshops, presentations and performances by Simon Baker, Curator of Photography at the Tate Modern, Antony Cairns, as well as «Selfie Stick Aerobic» by Arvida Byström and Maja Malou Lyse, «The Smoke House» by Melinda Gibson.

A high affinity binding site for HP1 can be produced by silencing Lys9 of histone H3 by methylation with mammalian histone lysing methyltransferase, a suppressor of variegation 39H1 (SUV39H1).