Sentences with phrase «lysed in»

Confluent 10 cm plates were fixed with 1 % PFA at room temperature for 10 minutes, lysed in SDS lysis buffer (50 mM Tris, 10 mM EDTA, 1 % SDS, Roche Complete protease inhibitors, pH 8.1), scraped and collected into 1.5 mL microcentrifuge tubes, and DNA was sonicated to 200 — 800 bp fragments with a Branson Sonifier 250 set to 30 % power / 90 % duty, four 10 second pulses.

Cultured cells were lysed in radioimmunoprecipitation assay (RIPA) buffer (50 μl RIPA per 1 × 106 cells; 50 mM Tris / HCl; 150 mM NaCl, 1 % Triton X-100, 1 mM EDTA, 1 mM NaF, 1 mM Na3VO4, 1 μg / ml leupeptin and 1 μg / ml pepstatin) for 1 h on ice, and insoluble debris was removed by centrifugation.

For this protocol, samples were lysed in 1 × RIPK2 lysis buffer [50 mM Tris (pH 7.5), 0 mM MgCl2, 1 % Triton X-100, 1 mM dithiothreitol, 1 mM EDTA, 1 mM EGTA, with freshly added 1 mM β - glycerophosphate and protease inhibitors (phenylmethylsulfonyl fluoride and aprotinin)-RSB- and immunoprecipitated overnight using 1.5 μg rabbit anti-RIPK2 antibody.

For Western blot analysis, 106 BaF3 cells were starved for 4 h and lysed in 250 μL of Laemmli buffer (BioRad).

NHLF cells transfected with siRNA molecules were lysed in High Salt ELB lysis buffer [1 M Tris pH 8.0, 1 % NP - 40, 250 mM NaCl, 5 mM EDTA] supplemented with protease and phosphatase inhibitors (1x G - Biosciences Protease Arrest, 200 µM Na3VO4, and 1 mM PMSF).

Cells were lysed in radioimmunoprecipitation assay buffer with protease and phosphatase inhibitors.

Cells were lysed in RIPA lysis buffer.

Cells were washed once with PBS and lysed in 0.02 mM NaOH for 16 hr at 4 °C, and then stored at — 20 °C until analysis.

At 48 hours, cells were washed twice with PBS and lysed in 1 × passive lysis buffer (Dual - Luciferase Reporter Assay, Promega).

Not exact matches

In a new report, the BBC reporter Lyse Doucet and camera operator Philip Goodwin described the atmosphere at the luxury hotel as «very serious.»

Lysing the red blood cells diminishes the tendency of blood to clog the system, a common problem that slows processing time in similar CTC filtering devices.

We have designed a microfluidic device in which we can manipulate, lyse, label, separate, and quantify the protein contents of a single cell using single - molecule fluorescence counting.

The distinction between apoptosis and cell lysis is important in immunology - lysing a virus - infected cell would only release the virions, whereas apoptosis leads to destruction of the virus inside.

In addition, it was increasingly recognized that oncolytic viruses not only were able to directly lyse cancer cells, but they also «freed «tumor specific neoantigens, indirectly acting as a cancer vaccine.

Cells were then lysed and HMOX1 mRNA was detected using the Quantigene II method as described in Materials and Methods.

In preclinical research, tumor cells lysed by coxsackievirus A21 (CVA21), a naturally occurring «common cold» ICAM - 1 — targeted RNA virus, induced a secondary systemic host - generated antitumor immune response.

Expression of four stem cell marker genes normalized to cyclophilin in nine single cell equivalents prepared from a pool of nine lysed HES3 cells.

T - VEC encodes GM - CSF, and is thought to replicate in tumor cells, lysing cells in injected tumors.

If the red blood cells fracture (lyse, hemolized) while they are withdrawn from your pet's vein or in tube transfer, LDH levels will also go up falsely.

The donation was made in honor of Dr. Carol Rhodes and Lyse O'Donnell Rvt and all of the staff at the Wentzville Veterinary Clinic who gave her such loving care.

Additionally, hemolysis results in release of analytes and enzymes from red blood cells (RBCs), which can falsely elevate many test results.5 Destruction of RBCs yields a lower RBC count.1 Excess fluid released from the lysed RBCs also creates a dilution effect in serum and can result in an artifactually decreased packed cell volume.