Sentences with phrase «lysing cells»

T - VEC encodes GM - CSF, and is thought to replicate in tumor cells, lysing cells in injected tumors.

One device segregates the cells into individual gel droplets, allowing researchers to lyse the cells and amplify their DNA while keeping them separated.

Additionally, most often detection is carried out on lysed cell samples, so a significant amount of information is lost.

The usual way is to lyse the cell, or break it open so that its contents spill out.

PC3 and PC3 DAXX K / D cells were processed using Power SYBR Green Cells - to - Ct Kit (Ambion, Cat # 4402953) to lyse cells, generate cDNA, and perform Q - PCR per the manufacturer's instructions.

Antigen - presenting cells then take up the lysed cells.

Not exact matches

EGFR / WT and KO cells were cultured overnight and starved for 24 h. Cells were treated with capsaicin (50 μmol / L) for 30 min and then TPA (20 ng / mL) for 2 h. Cells were lysed and cox - 2 mRNA levels were analyzed by RT -cells were cultured overnight and starved for 24 h. Cells were treated with capsaicin (50 μmol / L) for 30 min and then TPA (20 ng / mL) for 2 h. Cells were lysed and cox - 2 mRNA levels were analyzed by RT -Cells were treated with capsaicin (50 μmol / L) for 30 min and then TPA (20 ng / mL) for 2 h. Cells were lysed and cox - 2 mRNA levels were analyzed by RT -Cells were lysed and cox - 2 mRNA levels were analyzed by RT - PCR.

EGFR / WT and KO MEFs were treated with capsaicin (0, 10, or 50 μmol / L) for 30 min followed by TPA (20 ng / mL) for 4 h. Cells were lysed and COX - 2 expression analyzed by Western blot.

Cells were lysed and membrane and cytosolic fractions separated and EGFR abundance determined by Western blot.

Lysing the red blood cells diminishes the tendency of blood to clog the system, a common problem that slows processing time in similar CTC filtering devices.

We have designed a microfluidic device in which we can manipulate, lyse, label, separate, and quantify the protein contents of a single cell using single - molecule fluorescence counting.

The distinction between apoptosis and cell lysis is important in immunology - lysing a virus - infected cell would only release the virions, whereas apoptosis leads to destruction of the virus inside.

The metallopolymer by itself even demonstrated antimicrobial properties, lysing bacterial cells while leaving human red blood cells unaffected.

An examination into the host's immune response to symbiont presence suggests that firebugs actively harvest their bacterial partners by lysing, or bursting open, the bacterial cells.

At 48 hours, cells were washed twice with PBS and lysed in 1 × passive lysis buffer (Dual - Luciferase Reporter Assay, Promega).

Spleen cells (3 × 107) from either experimental or control effector lymphocytes were dispensed into 75 - cm2 tissue culture flasks along with 20 ml of boosting medium and 6 × 105 γ - irradiated (3,000 rad) BALB / c spleen cells (erythrocytes lysed).

Cells were lysed on ice using lysis buffer (10 mM Tris - HCl pH 7.4, 150 mM NaCl, 1 % Triton X-100, protease inhibitor mix G (Serva) and 2 mM Na3VO4).

Cells were washed once with PBS and lysed in 0.02 mM NaOH for 16 hr at 4 °C, and then stored at — 20 °C until analysis.

Cells were lysed in RIPA lysis buffer.

In addition, it was increasingly recognized that oncolytic viruses not only were able to directly lyse cancer cells, but they also «freed «tumor specific neoantigens, indirectly acting as a cancer vaccine.

Cells were lysed in radioimmunoprecipitation assay buffer with protease and phosphatase inhibitors.

NHLF cells transfected with siRNA molecules were lysed in High Salt ELB lysis buffer [1 M Tris pH 8.0, 1 % NP - 40, 250 mM NaCl, 5 mM EDTA] supplemented with protease and phosphatase inhibitors (1x G - Biosciences Protease Arrest, 200 µM Na3VO4, and 1 mM PMSF).

Cells were lysed with TN1 lysis buffer (50 mM Tris, 125 mM NaCl, 1 % Triton X-100, 10 mM EDTA, 10 mM sodium fluoride and 10 mM sodium pyrophosphate) supplemented with protease inhibitor cocktail (1.2 mM AEBSF, 0.46 μM aprotinin, 14 μM bestatin, 12.3 μM E-64, 112 μM leupeptin, 1.16 μM pepstatin)(Amresco; Solon, OH)-RRB- and 1 mM sodium orthovanadate (Enzo Life Sciences; Farmingdale, NY).

Cells were then lysed and HMOX1 mRNA was detected using the Quantigene II method as described in Materials and Methods.

NK cells are able to lyse virally infected cells and secrete large amounts of proinflammatory cytokines / chemokines without prior antigen sensitization.

Cells were lysed with 150 μl / well of RIPA buffer.

For Western blot analysis, 106 BaF3 cells were starved for 4 h and lysed in 250 μL of Laemmli buffer (BioRad).

Cell supernatants were discarded and cell pellets were lysed of RBCs and then used for staining with antibodies for flow cytometry analyCell supernatants were discarded and cell pellets were lysed of RBCs and then used for staining with antibodies for flow cytometry analycell pellets were lysed of RBCs and then used for staining with antibodies for flow cytometry analysis.

Cells were then lysed and probed for transcription of Nrf2, Keap1, or Bach1 using the QuantiGene II RNA plex (Affymetrix).

Cells were then lysed and centrifuged to pellet the nuclei.

Cells were lysed 48 h later with modified radioimmunoprecipitation assay buffer (RIPA buffer, 50 mM Tris - HCl, pH7.4, 150 mM NaCl, 1 % Nonidet P - 40 (NP - 40), 0.25 % sodium deoxycholic acid, 1 mM ethylenediaminetetraacetic acid (EDTA), 1 mM phenylmethylsulphonyl fluoride (PMSF), 1X Complete cocktail protease inhibitor (Roche, Penzberg, Germany)-RRB-.

(E) 106 BaF3 cells stably transduced with JAK2 V617F, Y931C and V617F / Y931C double JAK2 mutant were treated for 30 min with 300 nM CMP6 inhibitor or with DMSO as a control (− condition), lysed and subjected to Western blot analysis.

Cultured cells were lysed in radioimmunoprecipitation assay (RIPA) buffer (50 μl RIPA per 1 × 106 cells; 50 mM Tris / HCl; 150 mM NaCl, 1 % Triton X-100, 1 mM EDTA, 1 mM NaF, 1 mM Na3VO4, 1 μg / ml leupeptin and 1 μg / ml pepstatin) for 1 h on ice, and insoluble debris was removed by centrifugation.

(B) BaF3 cells stably transduced with V617F, Y931C or V617F / Y931C mutant were lysed and subjected to Western blot analysis.

(A) 106 BaF3 Aut (V658I), Aut (F958V) and BaF3 cells stably transduced with M - RAS or (B) JAK1 V658I and F958V mutants were treated for 30 min with 500 nM CMP6 inhibitor or with 0.1 % DMSO as a control (− condition), lysed and subjected to Western blot analysis.

The cells were lysed with RIPA buffer.

Isolation of cells from outer, middle and inner colony zones was performed under microscopic guidance (Figure S4), after which cells were immediately lysed and prepared for Q - RTPCR.

48 hours after transfection, cells were lysed with 100 µL / well 1 × passive lysis buffer (PLB, Promega) for 15 minutes with shaking.

Cells were washed with PBS three times and lysed by adding a modified RIPA buffer (50 mM Tris / HCl pH 7.4; 150 mM NaCl, 1 mM EDTA, 1 % Igepal, 0.25 % Na - deoxycholate).

In preclinical research, tumor cells lysed by coxsackievirus A21 (CVA21), a naturally occurring «common cold» ICAM - 1 — targeted RNA virus, induced a secondary systemic host - generated antitumor immune response.

Expression of four stem cell marker genes normalized to cyclophilin in nine single cell equivalents prepared from a pool of nine lysed HES3 cells.

Cells were washed with ice - cold PBS, lysed for 15 minutes on ice with M - PER protein extraction reagent (Pierce), scraped, and spun at 14,000 rpm for 10 minutes at 4 °C.

We believe that when G100 is administered with mechanisms that kill, or lyse, tumor cells (such as radiation), it may cause dendritic cells near the lysed tumor to activate and capture the wide range of released endogenous tumor antigens.

After 24 h, cells were incubated with or without PP2, lysed, and immunoprecipitated with anti-HA (left) or anti-myc (right) or control Abs (ctr Ab) followed by Western blotting with the indicated Abs.

WT and Lpar2 - / - T cells were incubated with 1 μM LPA for the periods indicated, and were lysed immediately thereafter.

Supplies Serratia peptidase enzyme to help protect sensitive GI tissues from irritation and lysozyme for its unique lysing action on bacterial and yeast cell walls

Yes, but it doesn't lyse fat cells as effectively as niacin.

If the red blood cells fracture (lyse, hemolized) while they are withdrawn from your pet's vein or in tube transfer, LDH levels will also go up falsely.

Additionally, hemolysis results in release of analytes and enzymes from red blood cells (RBCs), which can falsely elevate many test results.5 Destruction of RBCs yields a lower RBC count.1 Excess fluid released from the lysed RBCs also creates a dilution effect in serum and can result in an artifactually decreased packed cell volume.