Sentences with phrase «mesc pluripotency marker»

Whitehead Institute researchers have determined that the transcription factor Nanog, which plays a critical role in the self - renewal of embryonic stem cells, is expressed in a manner similar to other pluripotency markers.

We also interrogated the population of sorted, CD81 - negative cells for the expression of pluripotency markers (Figure 4, Panel B) and identified that successfully edited hiPS cells were 92.6 % Oct ‑ 4 positive, 99.7 % TRA ‑ 1 ‑ 60 positive, and 99.99 % SSEA - 4 positive.

Individual, edited (CD81 knockout) hiPS cells were expanded into clonal lines and analyzed for expression of CD81 and three pluripotency markers via flow cytometry using antibodies against CD81, Oct ‑ 4, TRA ‑ 1 ‑ 60, and SSEA - 4.

These data show that pluripotency markers in the expanded clones are maintained at levels comparable to those in the parental line, ChiPSC18.

Control and gesicle - treated hiPS cells were assessed for AcGFP1 expression (green), labeled with an antibody specific to pluripotency marker Oct ‑ 4, visualized with a fluorescent - labeled secondary antibody (red), and nuclear - labeled with DAPI (blue).

Panel B. CD81 - negative cells were examined for pluripotency markers Oct ‑ 4, TRA ‑ 1 ‑ 60, and SSEA - 4.

To determine pluripotency and AcGFP1 knockout efficiency in gesicle - treated cells, cells were labeled with a fluorescently labeled antibody specific to the pluripotency marker SSEA - 4.

Pluripotency was maintained in all edited clonal lines, as evidenced by the persistent expression of the three pluripotency markers.

After, cells were analyzed by flow cytometry or immunocytochemistry for AcGFP1 and pluripotency markers.

RT - PCR analysis of RNA samples from EBs on days 3, 6 and 9 revealed a gradual decrease in expression of endogenous pluripotency markers such as Nanog, Rex1, Oct4 and Sox2 as compared to their expression in undifferentiated iPS cells (Figure 4B, upper panel).

(C) RT - PCR results using specific primers against transgenic 4 factors and endogenous pluripotency markers.

Furthermore, data from RT - PCR analysis showed that transgenic 4 factors were completely silenced and endogenous pluripotency markers Oct4, Sox2, Rex1 as well as Nanog were successfully activated (Figure 3C).

All iPS lines (iPS1 - iPS3) showed the full complement of pluripotency markers utilized.

(A) Expression of pluripotency markers from iPSCs (HiPSC - 1 control) generated following retroviral transduction of unsorted HUF1 cells.

SSEA3 is a cell surface glycosphingolipid considered an embryonic / pluripotency marker [14], [15].

(A) Expression of pluripotency markers on H9 ESCs and SSEA3 - selected HiPSC lines.

Towards the center of the colony, ∼ 40 % of the cells had lost expression of all pluripotency markers, and we found a number of cells that were null - expressing no markers of pluripotency or lineage commitment, only the housekeeping gene cyclophilin a. However, many cells in this region still expressed Oct - 4.

The lack of co-expression of pluripotency markers and lineage specific markers at the protein level despite robust co-expression of transcripts of both classes of gene suggests a role for post-translational regulation of expression, perhaps by small RNAs.

To our knowledge, this study is the first to identify a pluripotency marker in a heterogeneous population of human dermal fibroblasts, to isolate a subpopulation of cells that have a significantly increased propensity to reprogram to pluripotency and to identify a possible mechanism to explain this differential reprogramming.

When five lines were further expanded and characterized, all demonstrated expression of key pluripotency markers expressed by hESCs, which included: alkaline phosphatase, Nanog, SSEA3, SSEA4, TRA160 and TRA181 (Figure 5A).

They showed pluripotency in many ways, including their expression of pluripotency markers, such as OCT3 / 4, NANOG, SOX2, SSEA1, SSEA4, and AP in immunofluorescence assay, Oct4, Nanog, Sox2, Klf4 and cMyc in RT - PCR.

Not exact matches

Colonies of genetically corrected cells taken from Fanconi anemia patients show red and yellow, markers associated with pluripotency.

Among the hundreds of colonies that grew from these cells, the scientists found that a handful had markers for pluripotency.

They were also able to express markers of the three germ layers, suggesting that they re-established pluripotency at the molecular and cellular levels (Fig. 2I).

Quantitative PCR analysis was performed to compare transcript levels of Venus with PrEn (Hex, Gata4, Gata6, Dab2, Sox7, Hnf4α, and Pdgfrα), pluripotency (Nanog, Klf4, Rex1, Stella, and Pou5f1) and other lineage (T, Fgf5, Eomes, Flk1, Mixl1, Cdx2, Sox1, Pax6, and Six3) markers in purified cell fractions.

(C) Gene expression changes characteristic of PrEn, ICM / pluripotency, neurectoderm, and mesoderm genes (expression of individual markers are included as supplementary, Figure S3).

Panel illustrates marker genes implicated in pluripotency of NSCs, with color bar reporting log2 normalized expression values (green / red indicates high / low relative expression).

Panel below illustrates marker genes implicated in pluripotency of NSCs, with color bar reporting log2 normalized expression values (green / red indicates high / low relative expression).

As seen in cells dissected from colonies, significant numbers of cells coexpressed lineage specific markers along with pluripotency genes.

In the present study, we again observed a continuous gradient in the expression of pluripotency genes across the cell populations that paralleled the gradient in cell surface marker expression.

A more recent study showed overlapping expression of nanog with GATA - 6 and a Pdgfra reporter, markers of the primitive endoderm lineage, from the morula to the 64 cell stages [8], suggesting a gradual transition from a pluripotency program to a committed state.

Finally, within the fractionated cell populations, there was evidence for coexpression of lineage specific markers alongside of pluripotency genes, similar to lineage priming described first in hematopoietic stem cells [15].

Cells along the continuum show a progressively decreasing likelihood of self renewal as their expression of stem cell surface markers and pluripotency genes wanes.

Oct - 4 is most consistently expressed of the pluripotency genes that we have studied, and it is switched off only in populations that have lost other measurable features of pluripotency, such as stem cell surface marker expression and the capacity for self - renewal.

We only found cells expressing neural markers within the central zone, where many cells were negative for both pluripotency and lineage specific markers.