Sentences with phrase «mm citrate»

Tissue samples were fixed by perfusion with paraformaldehyde, permeablized with 0.5 % Triton X-100 and blocked with 10 % serum for 1 hour at 25 °C; antigen retrieval was by heat mediation in 10 mM citrate buffer, pH 6, for 20 minutes at 97 °C in a water bath.

Tissue sections were processed with 10 mm citrate buffer (pH 6.0) in a microwave (100 °C; 15 min), although in some cases 10 - min pre-trypsinization was also required.

Not exact matches

In brief, STZ (Sigma - Aldrich) was diluted in sodium citrate buffer (10 mM, pH 4.5) and injected i.p. at 110 mg / kg per day for a consecutive 2 d. 1 mo after treatment, pancreas from fasted animals with a blood glucose level > 500 mg / dL was collected and used as inoculum for i.p. injections.

Slides containing a mixture of DU145 and 22Rv1 cells (see Figure S1, Table S1, Table S2) were pretreated in 2 × SSC (0.3 M NaCl, 0.03 M sodium citrate, pH 7.0 (Invitrogen)-RRB- at 73 °C for 2 min, followed by a 10 - min incubation in pepsin solution (0.5 mg / ml pepsin in 10 mM HCl; USB, Cleveland, OH) at 37 °C.