Sentences with phrase «mm potassium phosphate»

Embryos and EBs were washed in PBS solution (80 mM sodium phosphate, 15 mM potassium phosphate, 27 mM KCl, and 1.37 M NaCl), then fixed with X-gal fix solution (1 × PBS, 2 mM MgCl2, 5 mM EGTA, 1 % paraformaldehyde, 0.2 % Glutaradehyde, 0.02 % NP - 40) at 4 °C for 20 min.

The assay mixtures were prepared in 25 mM potassium phosphate buffer pH 7.5, 1 mM EDTA, 2 mM MgCl2, 0.01 % Triton X-100 with 5 µM SAHH, 0.3 U / ml of adenosine deaminase from Sigma, 25 µM SAM, and 15 µM Thioglo - 1.