Sentences with phrase «mm sodium tetraborate»

Brains were fixed with 4 % paraformaldehyde in 100 mM sodium tetraborate, pH 9.5, for 3 h, cryoprotected with 20 % sucrose - potassium - PBS (KPBS), and sectioned into coronal (30 μm) sections using a sliding microtome (Leica Microsystems Inc, Buffalo Grove, IL, USA).