Sentences with phrase «mrna expression levels»

ACTB mRNA expression levels in lymphoblastic lines and fibroblasts derived from affected individuals were decreased in comparison to those in control cells.

Overall, parthenotes have similar mRNA expression levels as IVF / ICSI embryos at the blastocyst stages (Figure 3 and 4B).

We also observed that parthenotes from in vitro matured oocytes have similar mRNA expression levels of the majority of studied genes compared to IVF / ICSI embryos at the blastocyst stage.

Notably, a positive correlation between the mRNA expression levels of DDX3 and p21waf1 / cip1 in 45 HCC specimens was also found by linear regression analysis (r = 0.501, P < 0.001; Fig. 5D).

E: Comparison of mRNA expression levels in adipose tissue from nonobese (BMI < 30 kg / m2, n = 40) and obese (BMI > 30 kg / m2, n = 30) NGT subjects.

High mRNA expression levels of HNF4α and ASGPR1 are shown in the enhanced hiPS - HEP cells for an extended culture time and are comparable to levels in cryopreserved human primary hepatocytes (hphep cells) that have been grown for only a short time.

cDNA synthesized by reverse transcription of 1 µg RNA primed with oligo (dT) and random 9 - mer primers was used as the substrate for quantitation of mRNA expression levels by quantitative RT — PCR in the presence of SYBR ® Green (Stratagene, La Jolla, CA).

Consistent with low mRNA expression levels, neither the Xpd † XPCS nor the Xpd † XP allele was able to restore TFIIH abundance to wt levels in XpdTTD compound heterozygote cells (Figure 4E and 4F).

Alterations of methylation status and mRNA expression levels of RARβ, MGMT, p16, or hMLH1 genes after treatment with EGCG or other polyphenols.

For each gene, the top panel presents the unmethylation - specific bands (UMSB), the middle panel presents the methylation - specific bands (MSB), and the bottom panel presents the mRNA expression levels (mRNA).

The average mRNA expression level of morulas (A) and blastocysts (B) from parthenogenesis and IVF / ICSI were displayed.

Not exact matches

To investigate, they measured mRNA levels associated with the expression of 23,000 genes in human brain tissue.

Unlike most biomarkers for cancer, DDX3 mRNA expression only increased slightly in the exposed breast cells, but the corresponding proteins levels were significantly higher.

The expression of mRNA for factors involved in promoting mitochondrial biogenesis, including the transcription factor Ppard, the PPARδ coactivator Pgc - 1α, and citrate synthase was greater in gastrocnemius muscles from IL - 15Rα — KO relative to B6129 control (Figure 5C); however, levels of these genes were unchanged in spleen and kidney (data not shown).

In addition, gastrocnemius muscles from IL - 15Rα — KO mice expressed higher levels of mRNA for the sarcoplasmic endoplasmic reticulum calcium ATPase - II (SERCA2) and lower mRNA expression and protein content of the calcium - binding protein calsequestrin (Figure 5, F and G).

Besides, researchers have also collected very large molecular datasets linked to gene expression in different organs of BXD mice, such as levels of mRNA, proteins, and metabolites.

The muscles from IL - 15Rα — KO mice had a greater relative expression of the SERCAII isoform and lower mRNA and protein levels of calsequestrin, both characteristic of slow muscles (52).

For example, hypoxia has been reported to induce VEGF expression in the retina, both at the mRNA [16,17] and protein levels [16].

In contrast, the level of XPCS mRNA expression did affect the ability of the encoded protein (XPDG602D) to restore the TTD hair phenotype to normal.

Despite reduced levels of mRNA expression, the homozygous lethal Xpd † XPCS allele ameliorated multiple XpdTTD - associated disease symptoms in compound heterozygous XpdTTD / † XPCS animals including the hallmark brittle hair and cutaneous features fully penetrant in homo - and hemizygous TTD mice (Figure 2A — 2C).

The defect in SMN2 gene expression in SMA patients is at the level of premRNA splicing, such that exon 7 tends to be left out of the mRNA that ultimately makes SMN protein.

mRNA expression from the targeted allele could be detected in embryonic stem cells by RT - PCR (Figure 1D), although expression was reduced approximately 5-fold relative to wt mRNA transcript levels as determined by Northern blotting of RNA from the testis of heterozygous animals (Figure 1E).

Finally, mRNA expression of striatal tissue found no differences in BDNF expression although expression of TNF - in the 3 - NP - iPSC - 21 and -42 rats had significantly lower levels than sham control and 3 - NP - iPSC - 7 rats, suggesting that there was not a continuous immune response to iPSCs.

Expression levels of DDX3 mRNA in HCC samples as shown in (B) are classified into unchanged, decreased, and enhanced categories.

The expression levels of AtRCCR and AtChlase from the same PlantPho promoter differ, possibly from differences in mRNA stability for AtRCCR and AtChlase.

Although there was no effect in the null mutant, we found ilp2 mRNA expression increased and AKT expression level was down regulated in larvae where either TOR signaling activity or the expression of GBP1 and GBP2 was specifically reduced in the fat body.

Proteomics — the large - scale study of proteins, particularly their structure and function — combined with transcriptomics — the examination of the expression level of messenger ribonucleic acids (mRNAs) in a given cell population - played a significant role in the recent derivation of a combined protein and gene list that constitutes one of the largest descriptive data sets of its kind.

Silencing of Nrf2 significantly decreased baseline levels of HMOX1, whereas silencing of Bach1 resulted in a 50-fold increase in expression of HMOX1 mRNA (Figure 4B).

As shown in Fig 5D, dilp5 mRNA levels were significantly reduced in CCHa2 - knockdown larvae, indicating that peripheral tissue - derived CCHa2 activates dilp5 expression in the brain.

Specifically, a mouse paradigm that mimics binge alcohol drinking in humans produced a robust reduction in BDNF mRNA levels in the medial PFC (mPFC), which was associated with increased expression of several miRNAs including miR - 30a - 5p.

His group uses high - throughput genomic methods — including DNA sequencing, genotyping, chromatin immunoprecipitation, mRNA expression profiling, transcriptional promoter and DNA methylation measurements — as well as computational and statistical tools to identify, characterize and understand the functional elements encoded in our genomes and how they work together at the molecular level in normal and pathological conditions.

To directly measure the level of coordination in the expression of genes, we used highly sensitive fluorescence in situ hybridization (FISH) to count individual mRNAs of functionally related and unrelated genes within single Saccharomyces cerevisiae cells.

mRNA molecules contain the instructions for making proteins — the number of mRNAs from a given gene is a measure of the level of expression of that gene.

Kayla Coldsnow, a Rensselaer doctoral student and the first author on the study, tracked the expression of the mRNA of PER in Daphnia exposed to naturally low salt levels and constant dark conditions.

Interestingly, while high levels of fluorescence and the expression of Hex and Cerberus mRNA required activin, low levels of Venus fluorescence were detected in the absence of activin.

The detection of this level of Venus expression in the presence of low levels of Hex mRNA suggests that this reporter is indeed extremely sensitive to the low levels of Hex transcript produced in the absence of activin, earlier in differentiation, and in undifferentiated ES cells.

Home > Press > Single - cell mRNA cytometry via sequence - specific nanoparticle clustering and trapping: Cell - to - cell variation in gene expression creates a need for techniques that can characterize expression at the level of individual cells

MEIS1 intronic risk haplotype associated with restless legs syndrome affects its mRNA and protein expression levels.

In some cases affected transcripts in Cre +; Tsc1 flox / flox mice showed significant variation among individuals that can not simply be explained by differential effectiveness of CRE - mediated knockout since, except one animal, all Cre +; Tsc1 flox / flox mice showed similarly reduced expression levels of Tsc1 mRNA.

(B) Liver expression of Apoc3 mRNA levels in mutant mice on a high - fat diet for 6 weeks and treated for the last 5 weeks with control or ApoC - III ASO (n = 3 — 7 / group).

Treatment of iLpldf mice with ApoC - III ASO decreased Apoc3 mRNA levels (Figure 8A) and protein expression (Supplemental Figure 6A) and reduced plasma TGs (Figure 8B) and TGs in CR / VLDL particles (Figure 8C).

Compared to PHWSC adipocytes, SGBS adipocytes showed not only greater induction of adipogenic gene expression during differentiation but also increased levels of UCP1 mRNA and protein expression.

Analysis of Oct4 protein expression in differentiated Ara - C cultures (DACs) showed a slight increase of Oct4 protein by day four of aggregate formation (Figure 1C), consistent with previous reports examining Oct4 mRNA dynamics [22] and the finding that primitive endoderm differentiation is accompanied by a transient increase in Oct4 protein levels [23].

Third, wild - type and toddler mutant embryos were injected at the one cell stage with increasing levels of Nodal mRNA and Nodal target gene expression was measured just prior to gastrulation using qRT - PCR.

The co ‐ expression of neighboring genes at the mRNA level is driven by chromatin fluctuations and direct regulatory interference.

Other studies at the molecular level have shown Berberine changes the expression of PPARgamma2 mRNA genes to inhibit formation of adipocytes (fat cells)(19, 20).

MuRF - 1 mRNA expression was elevated 2 h following exercise but had returned to basal levels, by 8 h in all treatments.

The Clock mutants were also shown to exhibit reduced overall expression levels and a blunted diurnal rhythm of orexin mRNA, a hypothalamic neuropeptide involved in energy regulation (2).

Robust changes in expression of brain - derived neurotrophic factor (BDNF) mRNA and protein across the brain do not translate to detectable changes in BDNF levels in CSF or plasma.