Sentences with phrase «magnetic bead cell»

Laboratory: Experience with laboratory applications in parasitology, immunology and molecular biology including, microscopy, BCA Protein Assay, ELISA, Flow Cytometry, Real Time qPCR, Luminex, Gas chromatography (GC) mass spectrometry, cotinine assays (saliva), magnetic bead cell separation (MACS, Percoll and Ficoll) and whole blood cultures.

Not exact matches

A team led by the Hutch's Marie Bleakley and by Warren Shlomchik of the University of Pittsburgh School of Medicine in Pennsylvania removes naïve T cells from grafts with a magnetic system that uses monoclonal antibodies bound to iron beads.

The kit adopts a novel purification method, using magnetic beads and phosphatidylserine - binding protein to isolate exosomes and other EVs from cell - culture medium and body fluids by a normal microcentrifuge.

Despite the ability of R3A to utilize both CCR5 and CXCR4 to infect cell lines, in human CD4 + T cells stimulated with anti-CD3 / anti-CD28 coated magnetic beads, CCR5 is downregulated causing transient resistance to R5 HIV [47].

The cells were stimulated with anti-CD3 / anti-CD28 coated magnetic beads at a 3 ∶ 1 bead to cell ratio [34].

Five days post-stimulation the anti-CD3 / anti-CD28 coated magnetic beads were removed from each of the three cultures (non-transduced (NTD), AdX4 - ZFNs, and AdR5 - ZFNs) and 2.5 million cells were seeded in each of four cultures that were subsequently infected with either Bk132 (primary X4 isolate), HxB2 (lab - adapted X4 isolate), R3A (R5X4 primary isolate), or media only (mock).

The putative DN cells were isolated by negative selection using anti-CD8 magnetic bead isolation followed by in vitro treatment with anti-CD8 antibody and complement.

Spleen cells were enriched for CD8 + and CD8 − T cells using magnetic beads.

After washing with PBS / 5 % FCS, stained cells were depleted by incubating the cell suspension with 500 μl Dynabeads magnetic beads (Life technologies, # 1141D) for 30 min at 4 °C on a rotator.

He still likes to tinker; only now he uses magnetic beads, atomic - force microscopes, and laser «tweezers» to explore the inner workings of the cell and the physical forces behind DNA replication.

For sorting human ILCs, tonsillar lymphocytes were depleted of B cells using CD19 magnetic beads (eBioscience).

With MagSi - Direct, you can start with a molecule of interest, attach magnetic beads to it and then use a magnet to isolate that molecule from any desired reaction mixture, together with any other molecules, complexes or even intact cells to which the starting molecule has bound.

We have a limited amount of product available for trial by users tired of the limitations of magnetic beads or who want to manipulate cells in a new way.

Cellular biology: Isolation and culture of various cell types; cryopreservation; Mixed Lymphocyte Reaction (MLR); cloning by limiting dilution; cytotoxicity assays using Chromium - 51 release; proliferation assay (CFSE and 3H - thymidine); ELISA; Elispot; magnetic beads separation.

High quality coating of magnetic silica beads with Steptavidin, Protein A, Protein G or other ligand specific molecules allows isolation of specific target molecules or cells out of large volumes or complex mixtures.

HIF -1-alpha was immunoprecipitated using 0.5 mg HeLa Nuclear DFO treated whole cell extract (ab180880), 5µg of Rabbit polyclonal to HIF1 alpha and 50µl of protein G magnetic beads (+).

HIF - 1 alpha was immunoprecipitated using 0.5 mg HeLa Nuclear DFO treated whole cell extract (ab180880), 5 µg of Mouse monoclonal to HIF - 1 alpha and 50 µl of protein G magnetic beads (+).