Sentences with phrase «magnetic beads»

We have a limited amount of product available for trial by users tired of the limitations of magnetic beads or who want to manipulate cells in a new way.

To diagnose malaria, for instance, a blood sample is fed into a closed plastic tube filled with millions of tiny magnetic beads coated with nickel.

So the researchers turned to a machine made by Quanterix of Lexington, Massachusetts, that uses magnetic beads coated by an antibody that binds selectively and extremely tightly to the protein in the blood.

The cells were stimulated with anti-CD3 / anti-CD28 coated magnetic beads at a 3 ∶ 1 bead to cell ratio [34].

First, IgE antibodies from the patient's blood are isolated by interaction with magnetic beads that are coated with a different type of antibody.

They added these beads to the sample to be tested, and then added that mixture to a test tube containing magnetic beads coated with DNA strands that bind the other half of the DNA.

A new technique based on magnetic beads is just as accurate as polymerase chain reaction (PCR) assays — the current gold standard for DNA detection — and could offer some new advantages for diagnostic tests and genetic research.

They injected a tiny magnetic bead into the nucleus and turned on a magnet to add a known force.

HIF -1-alpha was immunoprecipitated using 0.5 mg HeLa Nuclear DFO treated whole cell extract (ab180880), 5µg of Rabbit polyclonal to HIF1 alpha and 50µl of protein G magnetic beads (+).

Briefly, rhesus CD4 + T cells were isolated from whole blood, purified by magnetic bead negative selection, and then stimulated with anti-CD3 / anti-CD28 coated beads as previously described [35], [36].

Laboratory: Experience with laboratory applications in parasitology, immunology and molecular biology including, microscopy, BCA Protein Assay, ELISA, Flow Cytometry, Real Time qPCR, Luminex, Gas chromatography (GC) mass spectrometry, cotinine assays (saliva), magnetic bead cell separation (MACS, Percoll and Ficoll) and whole blood cultures.

Cellular biology: Isolation and culture of various cell types; cryopreservation; Mixed Lymphocyte Reaction (MLR); cloning by limiting dilution; cytotoxicity assays using Chromium - 51 release; proliferation assay (CFSE and 3H - thymidine); ELISA; Elispot; magnetic beads separation.

Following lysis and solid - phase capturing of mRNAs with streptavidin magnetic beads, samples were reverse transcribed using random primers.

The plasma containing magnetic beads then flows through the microchannels on the diagnostic chip in which the beads can be readily collected using a magnet to extract circulating exosomes from the plasma.»

Each is composed of building blocks bound together, like a string of small magnetic beads — and just as magnets have poles, these building blocks have «plus» and «minus» ends.

The opsonins inside this device are genetically modified variants of naturally occurring ones, each one stuck to the surface of a microscopic magnetic bead.

Given these different methylation patterns, the researchers used specialized magnetic beads that hybridize with methyl - CpG - rich DNA regions in an attempt to separate nuclear DNA from organellar DNA in total genomic DNA samples.

To bind the four micrometer - wide magnetic beads into pairs, Kim and his colleagues prepared two batches separately coated with avidin and biotin proteins, which create one of the strongest naturally found non-covalent bonds.

The putative DN cells were isolated by negative selection using anti-CD8 magnetic bead isolation followed by in vitro treatment with anti-CD8 antibody and complement.

DropArray offers three platforms that address unmet needs for cell processing with flow cytometry (DA - Cell), miniaturizes and improves magnetic bead - based assays (DA - Bead), and enables reduction of cells and expensive reagents for high content screening (DA - HCS).

Briefly, Flt3L - induced splenic DCs were isolated with CD11c magnetic beads and pulsed with LCMV peptides (2 μM) for 2 h. gp33 and NP396 purified peptide were obtained from GenScript.

For sorting human ILCs, tonsillar lymphocytes were depleted of B cells using CD19 magnetic beads (eBioscience).

After removal of the meninges, tissue was digested at 37 °C with 59 U / ml papain (Worthington) in Hanks balanced salt solution (HBSS, Invitrogen) supplemented with 10 mM Hepes (EMD), pH 8.0 and 125 U / ml Dnase I (Sigma), and triturated in 0.3 % (w / v) BSA / HBSS (Sigma), 250 U / ml Dnase I. A2B5 + PSA - NCAM − glial progenitor cells were isolated by step-wise immunopurification using anti-PSA-NCAM and A2B5 - bound magnetic beads (Miltenyi).

With MagSi - Direct, you can start with a molecule of interest, attach magnetic beads to it and then use a magnet to isolate that molecule from any desired reaction mixture, together with any other molecules, complexes or even intact cells to which the starting molecule has bound.

1 x 96 - well, includes coupled magnetic beads and detection antibodies for detecting TGF - β3, requires reagent kit (# 171-304070 for vacuum separation or # 171 - 304070M for magnetic separation) and a vial of standards (# 171 - X40001)

Based on 6.5 µm magnetic beads, it is compatible with both vacuum and magnetic wash stations.

Hiroshi Handa of the Tokyo Institute of Technology and his colleagues developed tiny magnetic beads — just 200 nanometers in diameter — that can be attached to drugs and other compounds.

Despite the ability of R3A to utilize both CCR5 and CXCR4 to infect cell lines, in human CD4 + T cells stimulated with anti-CD3 / anti-CD28 coated magnetic beads, CCR5 is downregulated causing transient resistance to R5 HIV [47].

AMSBIO has announced two new additions to its MagSi range of magnetic bead - based products for protein purification, proteomics, and genomics applications

Spleen cells were enriched for CD8 + and CD8 − T cells using magnetic beads.

DropArray ™ plates are compatible with magnetic beads.

The magnetic bead - based immunoassay panel facilitates detection of more samples than any other available assay, allowing researchers to get the greatest possible amount of data from each run.

Magnetic beads have long been used to isolate nucleic acids as well as recombinant proteins.

«We used magnetic beads of 3 micrometers in diameter to pull down the exosomes in plasma samples,» Zeng said.

The bound antibodies are then flushed out of the capillary and powerfully attached to the magnetic beads through a process called «crosslinking», which keeps them from detaching.

What's new in KatharoSeq is that Minich integrated a sample pooling strategy, appropriate positive and negative controls, high - throughput DNA extraction and a magnetic bead - based DNA clean - up approach.

Magnetic beads the size of a bacterium attach to specific surface proteins on white blood cells, and a magnetic field nudges these cells out of the stream of CTCs.

The magnetic beads were Ingber's idea, a remnant of work he'd begun at Yale.

Applying a magnet pulls out the magnetic beads, along with any linked gold beads and their associated bar - code DNA.

If the sequence of interest is in the sample, it forms a link between the gold beads and the magnetic beads.

Fixed to that plate are 300 million magnetic beads, and attached to each bead is a single molecule of DNA, which the machine is sequencing.

The kit adopts a novel purification method, using magnetic beads and phosphatidylserine - binding protein to isolate exosomes and other EVs from cell - culture medium and body fluids by a normal microcentrifuge.

Five days post-stimulation, the magnetic beads were removed and washed twice in fresh media.

Primary human CD4 + T cells were stimulated with anti-CD3 / anti-CD28 coated magnetic beads and transduced 18 hours later with AdX4 - ZFNs, AdR5 - ZFNs which expresses previously described CCR5 - specific ZFNs [23], or an Ad5 / F35 vector that expresses green fluorescent protein (AdGFP).

Five days post-stimulation the anti-CD3 / anti-CD28 coated magnetic beads were removed from each of the three cultures (non-transduced (NTD), AdX4 - ZFNs, and AdR5 - ZFNs) and 2.5 million cells were seeded in each of four cultures that were subsequently infected with either Bk132 (primary X4 isolate), HxB2 (lab - adapted X4 isolate), R3A (R5X4 primary isolate), or media only (mock).

Using a proprietary protocol DNA fragments are bound directly onto the surface of the magnetic beads, leaving unincorporated nucleotides, primers, primer dimers, and other contaminants in solution.