Sentences with phrase «mammary epithelial cell»

LARP7 was expressed at relatively high levels in the untransformed mammary epithelial cell lines (MCF10A and EpH4) and noninvasive breast cancer cell lines (MCF7, BT474, T74D, and ZR75B), but was markedly reduced in all four invasive and metastatic cancer lines examined (MDA - MB - 468, BT549, MDA - MB - 231, and MDA - MB - 435; Figure 1D).

(C) Degree of branching and number of mammary epithelial cell layers in the mammary tissue formed by mammary cells infected with miR - 150.

More specifically, he researches the use of anti-CEA antibodies for tumor imaging and therapy, as well as the role of CEACAM1 in T - cell activation and mammary epithelial cell polarization.

The human mammary epithelial cell line (HuMEC) was maintained in human basal serum - free medium (Life Technologies) with the HuMEC kit (Life Technologies).

Mammary epithelial cell phagocytosis downstream of TGF - β3 is characterized by adherens junction reorganization.

Differentiation of the mammary epithelial cell during involution: implications for breast cancer.

Prolactin - mediated regulation of lipid biosynthesis genes in vivo in the lactating mammary epithelial cell.

However, the regulatory mechanisms of mammary epithelial cell proliferation during pregnancy have been unclear.

As lipid droplets exit the mammary epithelial cell, they are enveloped by cell membrane and secreted into milk as membrane - bound globules of fat.

However, there is an intriguing workaround — human milk secreted during lactation is a rich source of mammary epithelial cell RNA.

About 3 — 8 % of human milk fat globules contain mammary epithelial cell cytoplasmic remnants, including RNA, captured during milk fat globule formation and secretion [9].

Transient Silencing of 14 -3-3sigma promotes proliferation of p63 - positive progenitor cells isolated from human breastmilk in mammary epithelial cell culture.

Milk sodium concentration is a marker of tight junction closure between mammary epithelial cells; with the onset of secretory activation (stage II lactogenesis) milk sodium concentrations decrease sharply as potassium concentrations increase [14].

Colostrum is characteristically high in sodium (> 30 mmol / L) and low in potassium (< 15 mmol / L) as a result of open paracellular pathways between mammary epithelial cells [14].

Secretion of the hormones estrogen and progesterone set the stage for dramatic changes that take place in the breast during pregnancy: a massive proliferation of mammary epithelial cells, and the formation of thousands of ductal structures, which support milk production and transport during lactation.

In cell culture, high expression levels of GPR161 induced proliferation of mammary epithelial cells, disrupted the acinar structures formed by these cells, and enhanced their invasive capacity.

In one experiment, the researchers created arrays of mammary epithelial cells and asked how adding one or more cells expressing low levels of the cancer gene RasG12V affected the cells around them.

When this model was in its softest mode, normal (benign) mammary epithelial cells behaved normally within it, forming cellular structures referred to as acini that capture many key features of the normal in vivo mammary epithelium.

«It is striking that these changes, found in many human cancers, can be induced in normal mammary epithelial cells simply by varying the stiffness or composition of the matrix surrounding them.»

HER2 - positive breast cancer, which is associated with poor prognosis, has been traced to an excess of signaling through receptors, or docking ports, called HER2 found on the surface of certain mammary epithelial cells.

«Two components of the pathway, the phosphatase PTPD2 and the lipid PA, are together required for HER2 signaling to function in mammary epithelial cells.»

To investigate, a team led by Gerburg Wulf, MD, PhD, a physician scientist in the Hematology / Oncology Division at Beth Israel Deaconess Medical Center (BIDMC) and an Associate Professor of Medicine at Harvard Medical School, cultured breast cells called primary mammary epithelial cells (MECs) in the presence of EBV.

The cells I had were ovine mammary epithelial cells: we were looking to increase expression of proteins in milk.

Collectively, these results suggest that Nrk negatively regulates the estrogen - dependent proliferation of mammary epithelial cells, the disruption of which leads to breast tumors.

The absence of tumors in virgin Nrk mutant mice strongly suggests that the tumorigenesis is closely related to the proliferation of mammary epithelial cells during pregnancy.

In Nrk mutant mammary glands, dense clusters of ER alpha - positive mammary epithelial cells were sometimes observed at late stages of pregnancy, which may represent the «bud» of the breast tumor.

In Nrk mutant mice, therefore, mammary tumorigenesis was proposed to be triggered by a combination of dysregulation of proliferation in mammary epithelial cells and excessive blood estrogen.

This suggests that Nrk is expressed in mammary epithelial cells late in pregnancy in order to terminate their proliferation.

During pregnancy, the elevated blood estrogen level induces the proliferation of mammary epithelial cells, leading to the development of the mammary gland in preparation for lactation.

Human breast cancer cells generated by oncogenic transformation of primary mammary epithelial cells.

Results: Researchers at Pacific Northwest National Laboratory have identified the secretome — defined as the family of proteins secreted by cells — of human mammary epithelial cells, or HMEC.

An instructive model of EMT is based on an experimental system employing normal primary human mammary epithelial cells (HMECs).

PI3K - Rac1 - JNK signaling mediates collagen I - induced cell scattering and up - regulation of n - cadherin expression in mouse mammary epithelial cells.

However, up to five-fold higher levels of activated matriptase were detected in the conditioned media from the cancer cells grown in the absence of serum and S1P, when compared to non-transformed mammary epithelial cells.

MMTV Env encodes an ITAM responsible for transformation of mammary epithelial cells in three - dimensional culture.

Using cutting - edge techniques enabled by next - generation sequencing, the authors generated complete methylome maps at single nucleotide resolution in a low - passage breast cancer cell line and normal breast tissue (primary human mammary epithelial cells).

Berkeley Lab scientists have developed a new method that can easily create immortal human mammary epithelial cells.

(2015) TRIM24 links glucose metabolism with transformation of human mammary epithelial cells.

Gene expression profiling of di - n - butyl phthalate in normal human mammary epithelial cells.

Moreover, ablation of a STAT5A allele reduces tumor incidence in a mouse model of breast cancer in which mammary epithelial cells express T antigen (48).

Characterising the hierarchy of mammary epithelial cells (MECs) and how they are regulated during adult development is important for understanding how breast cancer arises.

Last year, Mandriota and collaborators demonstrated that in a cancer mouse model, concentrations of aluminum in the amount of those measured in the human breast are able to transform cultured mammary epithelial cells, allowing them to form tumors and to metastasize.

Mammary epithelial cells were obtained as described previously (Stingl et al., 2006).

The MCF10A mammary epithelial cells were cultured in DMEM - F12 medium supplemented with 5 % horse serum, 20 ng / ml EGF, 10 μg / ml insulin, 0.5 μg / ml hydrocortisone, 100 ng / ml of cholera toxin, and penicillin / streptomycin.

However, when challenged by an oncogene, the normal mammary epithelial cells in these involuted glands did not aberrantly activate STAT5 (Figure 8A) when compared to mammary cells that gained caErbB2 first and were later exposed to pregnancy hormones (Figure 3A).

We note that our new results show that miR - 142 upregulates the expression of miR - 150 when miR - 142 is highly expressed and / or the Wnt signaling pathway is strongly stimulated in mammary epithelial cells.

And 2) to inhibit WNT signaling in breast cancer cells or mammary epithelial cells and test whether this leads to reduced miR - 150 expression.

We note that our results show that miR - 142 upregulates the expression of miR - 150 when miR - 142 is highly expressed and / or the WNT signaling pathway is strongly stimulated in mammary epithelial cells.

Considering that miR - 142 is highly expressed in human BCSCs, but weakly expressed or undetectable in the stem / progenitor population of the mammary epithelial cells, our result suggest that the upregulation of miR - 142 and its enhancement of the miR - 150 expression seem to be especially relevant in the breast tumor progression in vivo.

6) More importantly, the Figure 6 shows that when mammary epithelial cells are transfected with miR - 150 and grafted in empty mammary fat pads, they recapitulate the phenotype of activated Wnt signaling (largely hyperplastic mammary gland).