Sentences with phrase «many backcrosses»

· Molecular and Functional Characterization of GR2 - R1 Event Based Backcross Derived Lines of Golden Rice in the Genetic Background of a Mega Rice Variety Swarna — http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0169600

The researchers also report a lower Chlorophyll content in the backcrossed plants, which probably causes the pale green leaves.

A new study now finds, that backcrossing of the genetically engineered Golden Rice, with the Indian mega rice variety Swarna, leads to morphological changes such as pale green leaves, a reduced plant height and a drastic reduction in yield.

Progeny from the cross would then be crossed with the high - yielding parent to ensure that the progeny were most like the high - yielding parent, (backcrossing).

The resulting backcrossed plants and information about their genomes, «shows a way forward for improving chickpeas and many other crops too,» says von Wettberg, a professor in UVM's Department of Plant and Soil Science.

«How many backcrosses do we need?

The breeders hoped that a small proportion of each backcrossed generation would retain the high blight resistance of the Chinese trees while looking and acting more American.

In the 1980s, the American Chestnut Foundation launched a program in which Chinese - American hybrids were repeatedly backcrossed with American trees.

Authors should fully describe the source of their animals and number of times backcrosses were performed

A new study published this week in The Auk: Ornithological Advances shows that appearance alone is not enough to identify these hybrid zone birds: there is no single, intermediate «phenotype» or physical appearance common to all of the first - generation hybrids found, and birds from further backcrossed generations were often indistinguishable from the parent species.

Some closely related species interbreed where their ranges overlap, producing hybrid offspring that can even backcross with either parent species, until a whole population of mixed - species birds forms in the area and creates what's known as a «hybrid zone.»

Analyzing backcrossed maize - teosinte hybrids with molecular probes, Doebley's group came to a startlingly similar conclusion: The differences between maize and teosinte could be traced to just five genomic regions (5).

Likewise, previously observed increases in fruit fly lifespan associated with overexpression of dSir2, the equivalent to sir - 2.1 in flies, disappeared when Gems and colleagues backcrossed the modified flies with ordinary ones.

This process — known as backcrossing — helps to remove the influence of any other genetic differences.

A well - known breeding technique called backcrossing has become far more potent recently, as markers have allowed scientists to locate rare offspring that retain only the desired — and now detectable — genes from orphan crops.

With molecular backcrossing, Mackill, Ronald and their many colleagues were then able to breed this overexpressed gene into rice already popular in India, such as the legendary Swarna variety.

Previous attempts to backcross this trait with conventional breeding had always failed, reducing Swarna's taste or yield.

Then repeated backcrossing is undertaken to the elite line to fully recover this genetic background.

I was able to establish a transgenic line from one of these founders (F66), and I backcrossed this line extensively to C57 BL / 6 mice for subsequent analysis.

The mice in the original study were from an 8th generation backcross to the FVB line, thus it was on a mixed background, while the mice used in the replication will be from a pure 10th generation backcross to the FVB line.

Some closely related species interbreed where their ranges overlap, producing hybrid offspring that can backcross with either parent species, until a whole population of mixed - species birds forms in the area and creates what's known as a «hybrid zone.»

Each of these four transgenic lines was backcrossed at least 6 times to C57 BL / 6 mice prior to analysis in order to control for genetic background effects.

This spontaneously arising mutation has been maintained on a mixed genetic background (C57BL / J × C3Heb / FeJ - a / a × CD1) but recently has been backcrossed onto the FVB / NJ strain (57).

This may facilitate subsequent generations of backcrossing.

Mstn mutant and F66 and FLRG transgenic mice were backcrossed at least 6 times onto a C57 BL / 6 background prior to analysis.

New genes will be introduced from Oryza species through hybridization and backcrossing in elite parents.

• Speed congenics Traditional backcrossing requires a minimum of 10 generations, up to three years to complete, and uses five times the number of animals.

In 2015, Dr. Melissa R. Warden (Cornell University) purchased some Stock No. 010802 animals (total backcross generation N1 onto C57BL / 6J).

Of note, Dr. Warden's lab reports that, at least once during backcrossing, a heterozygous female was bred to a C57BL / 6J inbred male (thus the Y chromosome of the congenic strain is of C57BL / 6J origin).

For bulked segregant analysis, bsl1 - 1 plants were backcrossed to the reference line A10.1 as the pollen parent.

We can not rule out the influence of other SNPs fixed in the mutant populations on seemingly unrelated phenotypes, such as the elongated panicle phenotype displayed by bsl1 - 2 (Figure 1B), since several rounds of backcrossing were not performed.

Critical assessment of incoming strains — all submissions require detailed information about method of genetic manipulation, genotype and genotyping procedures, genetic background (e.g. source of strain and ES cell used in development, number of background generations and backcross parent strain), and reproducible phenotype.

(8) Imputation of these results specifically to the animals» age - related, low - BubR1 - driven rise in p16Ink4a - expressing senescent cells was, however, limited: limited by the very nature of so - called «accelerated aging» models such as BubR1H / H, (9) and limited by the lifelong, global absence of p16Ink4a expression in the backcrossed mice.

The technical evaluation of projects may require the provision of additional data such as information on the genetic modification of your mutant mouse line if applicable (e.g. affected gene, MGI ID of the gene, type of mutation, ES - cell line used, genetic background (e.g. number of backcross generations), safety level, description of DNA modification, vector, remaining non-recipient DNA, donor organism), mutant phenotype (s), special housing or care requirements, current sanitary status, and intellectual property rights (who generated the mouse line, owner of the mouse line)

DJ - 1 knockout mice were generated and originally characterized by Chandran and colleagues (2008) and given to us after having been backcrossed at least two generations into C57BL / 6J.

Male wing size increases by 45 % through cell size and cell number changes when the ws1 allele from N. giraulti is backcrossed into a N. vitripennis genetic background.

This permits movement of genes involved in phenotypic differences between the species by backcrossing [13]--[15].

One intriguing bit of work that Dr. Ley described was on the «Mouse APL» project, a knock - in mouse with the PML - RARA gene fusion backcrossed 10 + generations to CBL / BL6 mice.

We backcrossed the DJ - 1 mice for an additional three generations into C57BL / 6J prior to mating one DJ - 1 + / − mouse with one PolgWT / MT mouse.

To positional clone this major sex - specific wing QTL and to more precisely describe its phenotypic effects we (a) reduced the size of the introgressed sequence flanking the ws1 locus to a 40kb segment (see fine - scale mapping and cloning below) and (b) backcrossed the introgressed ws1g segment into a standard N. vitripennis strain (AsymCX) genetic background for > 10 generations.

All animals were fully backcrossed onto a C57Bl / 6 background.

In this study, we focused on two geographically distinct ivermectin resistant strains of H. contortus and two lines generated by multiple rounds of backcrossing between susceptible and resistant parents, with ivermectin selection.

Stock No. 005252 is an alternative strain, with a virtually identical genetic background except that it is wild - type for Pde6b, the result of continuous backcrossing to (C57BL / 6JEiJ x C3Sn.BLiA - Pde6b + / DnJ) F1 / J.

All mice were maintained after five generations of backcrossing to C57BL / 6J (B6) and housed under specific pathogen - free conditions in accordance with institutional guidelines.

Each of these strains has been backcrossed a limited number of times (Materials and Methods).

which originated from the 1955 breeding of a carpathian wolf and German shepard, and subsequently backcrossed again to wolves on three additional occasions within a 25 year period.

The diverse genetic origin of the domestic dog has likely involved multiple gray wolf populations from breed inception that were even at later times backcrossed with wolves throughout history.

Although there is less vigour in this generation than in the first, the second generation backcross still is close enough in the breeding tree to the original hybrid to still benefit from hybrid vigour.

She noted that those «backcrossed» dogs, which have been registered as Dalmatians with the United Kennel Club in the United States, offer a valuable resource for correcting the genetic defect that results in the elevated uric acid trait in the Dalmatian breed.

F1b — is a backcross in which a Bernedoodle is bred with a poodle.