«We use molecular biology and
cell biology techniques available today.»
BMBC168 is a shared platform for biochemistry, molecular biology and
cell biology techniques in our department.
Besides learning many
cell biology techniques (tissue culture, Westerns, generating DNA constructs, transfections) as well as fly genetics, students can get familiar with imaging techniques depending on their own interests and experience.
Recent progress in phylogenomics, and the implementation of modern molecular, microscopy, and
cell biology techniques in a handful of spiralian model systems have made that possible.
The projects will provide training in: current molecular biology techniques (including RNA interference, genetic transformation, analyses of gene expression);
cell biology techniques (cell culturing, cell transfections, imaging); protein biochemistry (2D electrophoresis, protein functional analyses); and bioinformatics (structure predictions, phylogenetic analyses; molecular interactions).
Our research strategy combines molecular and
cell biology techniques with live - cell imaging.
This course is aimed at researchers who are familiar with basic molecular and
cell biology techniques and want to learn how to create an engineered mammalian cell line using the most recent and advanced CRISPR / Cas9 system.
Not exact matches
Artificial wombs and embryos made from skin
cells — remarkable new
techniques could revolutionise reproductive
biology and help bring an end to infertility
What we are trying to do is introduce to
biology techniques normally used in chemistry or physics, using inherent chemical or structural properties of the human stem
cells.
University of Illinois researchers have developed a new
technique to create a
cell habitat of squishy fluids, called hydrogels, which can realistically and quickly recreate microenvironments found across
biology.
Similar to the naturally occurring effects of TET2 mutations in mice or humans, using molecular
biology techniques to turn off TET2 in mice caused abnormal stem
cell behavior.
Johnson's team borrowed a
technique from cancer
biology, called the systematic evolution of ligands by exponential enrichment (SELEX), which creates a huge library of random, short chains of nucleotides, called aptamers, and then incubates them with a target of choice, such a specific breast cancer
cell.
At the same time, the
techniques of genetics and molecular
biology began to reveal the intricate biochemical signals that synapses — the portals of nerve
cells — deploy during communication.
«The exciting part of this work is not just that we made hydrogels, but that we're now equipped with this powerful
technique that lets us ask fundamental — and very challenging — questions about them,» says Takanari Inoue, Ph.D., an associate professor of
cell biology at the Johns Hopkins University School of Medicine and senior author of the report on the research published online Nov. 6 in the journal Nature Materials.
Lead author Aaron Allen was a PhD student in
cell & systems
biology at U of T when the work was done, and he was assisted by Sokolowski, fellow EEB student Ina Anreiter, and Oxford University collaborator Megan Neville, who taught Allen the
technique.
An international team of researchers from Denmark and Russia used a series of biochemical and structural
biology techniques to investigate how the Qβ bacteriophage, which infects the common coli bacteria, utilises several of its host
cell's proteins while replicating its genetic material.
Co-first author, Ricard Argelaguet, a PhD student at The European Bioinformatics Institute (EMBL - EBI), said: «This
technique represents an important step towards a comprehensive characterisation of single -
cell biology.
The Salk and TSRI scientists used a range of neuro - genetic, gene therapy, biochemical and structural
biology research
techniques to discover that the mutant GlyRS enzyme blocked molecular signals important for maintaining the health of motor neurons, the
cells that carry messages from the brain to the muscles of the extremities.
«This study is a great example of a highly collaborative endeavor that combines expertise in systems RNA
biology, stem
cell and neuronal
biology, physiology, and cutting - edge imaging
techniques.
We worked with animal
cell cultures and molecular
biology techniques (and the occasional sheep if we were developing veterinary applications).
This
technique, which scientists are rapidly automating, may eventually displace crystallography as the tool of choice in many
cell biology studies because it does not require the subject to crystallize and sometimes can even reveal multiple conformational states in a single experiment.
In particular, they are now looking for expertise in molecular
biology, especially in virus - based
techniques for introducing new genes into specific
cell types in the grid -
cell circuit.
Using
techniques of molecular and
cell biology, transgenic models and clinical studies we will try to answer the following questions:
Noise has traditionally been difficult to study in
biology, because most biological
techniques, such as measurement of gene activity, average the output of many
cells, erasing individual variability.
The Human Emulation System creates an environment where
cells exhibit an unprecedented level of biological function, and gives researchers the ability to control complex human
biology and disease mechanisms that is not possible with other
techniques.
Expertise in in - vitro culture of respiratory
cell lines and primary human
cells, as well as knowledge of basic molecular
biology concepts and
techniques is a must.
This technology creates an environment where the
cells exhibit an unprecedented level of biological function, and provides control of complex human
biology and disease mechanisms not possible with existing
techniques.
By using a combination of genetics, fix and live imaging,
cell biology and molecular
biology techniques we are exploring the consequences of polyploidy in the brain and epithelial tissues of Drosophila.
The Innovator Award will enable Sipkins to continue to combine state - of - the - art imaging
techniques, performed on living mice, with
cell and molecular
biology approaches to learn more about these stem
cell niches.
The projects are multidisciplinary, involving a variety of
techniques including molecular
biology (PCR, cloning),
cell culture and advanced fluorescence microscopy.
Other structural
biology techniques, such as cryo - electron microscopy, can not resolve cellular elements beyond an intact
cell's surface, explains Julia Mahamid of the Max Planck Institute for Biochemistry, a member of the research team (Science 2016, DOI: 10.1126 / science.aad8857).
Although experimental mammalian genetics with the use of ES
cells and the
techniques pioneered by my co-awardees is now well founded and used, there is still much to be learned and much interesting research in store about what genes really do in the context of the real
biology of the whole organism in a complex environment.
• Knowledge of raw materials, quality control, production processes, and cost control • Familiar with the equipment and
techniques necessary for growing and harvesting raw ingredients and foods for human consumption • Proficient in U.S. Department of Agriculture USDA National Nutrient Database and PathogenTracker • Good understanding of
biology, especially animal and plant organisms and their
cells, functions, and interactions with one another • Excellent communication skills
Tags for this Online Resume: High - Throughput (HTS) and High - Content Screening (HCS), Research Scientist, Laser Scanning Confocal Microscopy and Spectrophotometry Usage and Analysis, Murine manipulation, • ELISA • Western Blot, Flow Cytometry, Microbiology and Immunology,
cell - based assays, molecular
biology techniques