In previous studies, Chen - Yu Zhang's group has reported a striking finding that plant miRNAs are ingested from plant diets and pass through the gastrointestinal tract, enter into the blood, accumulate in tissues and regulate
endogenous gene expression in animals.
The researchers also found that many
endogenous genes had additional repeating sections of A and T nucleotides that mark them as «self» and not foreign.
However, these assays are unsuitable for screening primary cells and can not assess impact on
endogenous gene expression.
Kim, Jung - Sik, et al. «Epitope tagging of
endogenous genes in diverse human cell lines.»
Amabile A, Migliara A, Capasso P, Biffi M, Naldini L, Lombardo A. Inheritable silencing of
endogenous genes by hit - and - run targeted epigenetic editing.
Workflow for targeted knockout of CD81,
an endogenous gene in hiPS cells, using the Cellartis iPSC CRISPR / Cas9 Gesicle and Single - Cell Cloning System.
Another way to dodge cellular variation observed in mammalian cells is to tag
an endogenous gene.
Targeted DNA demethylation and activation of
endogenous genes using programmable TALE - TET1 fusion proteins.
Efforts to tag
endogenous genes for proteins in these compartments using CRISPR systems should strongly consider using moxFPs over standard FPs for the reasons listed above.
These vectors can be used to tag
endogenous genes and simultaneously introduce a selection marker for screening modified strains.
Endo,
endogenous genes; tg, transgenes.
CRISPR has allowed scientists to tag and light up
endogenous genes of interest to better understand normal protein function.
Cas9 / gRNA can be easily engineered against almost
all endogenous genes within a 3 - day period of time, and goats carrying multiplex mutations can be generated within 5 months (gestation length 150 — 155 days).
Introduction of DNA double - strand breaks by site - specific endonucleases called meganucleases is being successfully used to target
endogenous genes in human cells.
Thus these results measure the relative rates of transcription for each gene; they are not a direct measure of mRNA accumulation or of protein abundance for
the endogenous gene products.
His pioneering mouse embryonic stem (ES) cell work in the 1980s, demonstrating germ - line transmission and the great potential of ES cells to generate mice carrying mutations in
endogenous genes, established milestones in a field that saw the award of the 2007 Nobel Prize in Physiology or Medicine to Mario Capecchi, Martin Evans, and Oliver Smithies.
Not exact matches
Perhaps the most significant distinction between evolution and ID / creationism is evolution's ability to explain poor design features, e.g. male nip - ples, the recurrent laryngeal nerve, the presence / location of
endogenous retroviruses, and (one of my personal favorites) the presence of a defunct
gene for egg yolk protein in our placental mammal genomes.
By using engineered zinc - finger nucleases (ZFNs) designed to target an integrated reporter and two
endogenous rat
genes, Immunoglobulin M (IgM) and Rab38, we demonstrate that a single injection of DNA or messenger RNA encoding ZFNs into the one - cell rat embryo leads to a high frequency of animals carrying 25 to 100 % disruption at the target locus.
Homologous recombination was used to introduce a nominally transforming mutation into an
endogenous H - ras1
gene in Rat1 fibroblasts.
To do so, the placenta may deploy a mercenary army of
endogenous retroviruses — viral
genes embedded in the mammal's DNA.
The roadmap outlines future research directions toward the goal of enhancing human radioresistance, including upregulation of
endogenous repair and radioprotective mechanisms, possible leeways into
gene therapy in order to enhance radioresistance via the translation of exogenous and engineered DNA repair and radioprotective mechanisms, the substitution of organic molecules with fortified isoforms, the coordination of regenerative and ablative technologies, and methods of slowing metabolic activity while preserving cognitive function.
Some of the first
genes to start expressing themselves again are former viruses turned into transposable elements known as human
endogenous retroviruses, particularly one called HERVK, Wysocka and colleagues found.
Analogous to integrated circuits that underlie myriad electronic products, engineered
gene circuits can be used to generate defined dynamics, rewire
endogenous networks, sense environmental stimuli, and produce valuable biomolecules.
Using the
gene - editing tool CRISPR, they snipped out several
endogenous viruses from the cell's DNA.
The more powerful one occurred within a stretch of DNA, or locus, that contains the HCP5
gene, which codes for a human
endogenous retrovirus — a genetic fossil of a virus that wove itself into human chromosomes long ago but no longer produces infectious progeny.
Because of these limitations, the AAV approach is best suited for tissue culture based approaches that require modification of only a single allele of a
gene, such as the introduction of heterozygous
endogenous epitope tags, or the introduction of dominant mutations of a
gene into the
endogenous allele in cultured cells.
Some examples from their lab include using AAV to introduce epitope tags into the
endogenous alleles of the p53 and PTEN tumor suppressor
genes in human cells (Kim et al 2008).
They have also used AAV
gene editing to introduce naturally occurring cancer - causing mutations into the
endogenous allele of the STAG2 tumor suppressor in human cells -LRB-
They have also used AAV
gene editing to introduce naturally occurring cancer - causing mutations into the
endogenous allele of the STAG2 tumor suppressor in human cells (Kim et al, 2016).
Kin Ritchie, Ralph Brinster, and Ursula Storb show that an IgL transgene inhibits rearrangement of
endogenous IgL
genes, providing the first clue for the mechanism of allelic exclusion of Ig
genes.
MiRNAs are small
endogenous noncoding RNAs which negatively regulate
gene expression.
After concluding from our proof - of - concept experiment that a successful gesicle - based knockout of a virally integrated
gene can be achieved, we chose to target CD81, an
endogenous membrane glycoprotein that forms complexes with integrins and plays a critical role in the infection process that leads to hepatitis C (Figure 3).
The Morris lab is specifically interested in utilizing the recently described
endogenous non-coding RNA pathway in human cells to epigenetically modulate
gene expression in those
genes involved in HIV, cystic fibrosis, and cancer.
In tobacco, we measured a reduction in the
endogenous POR
genes (NtPOR), and induction of the AtRCCR and AtChlase transgenes in response to TNT.
Notably, evidence suggests that this
endogenous pathway can be utilized to either epigenetically silence or activate a
genes expression.
Multiple mouse models of SMA have been produced, generally by knocking out the
endogenous mouse SMN1
gene and replacing it with one or more copies of the human SMN2
gene that produces much less full - length SMN protein than the SMN1
gene.
Figure 4D shows transcript levels for the
endogenous NtPOR
gene and the introduced AtChlase and AtRCCR
genes in the transgenic control plant (NT 4.1.3) and transgenic plants exposed to TNT.
Quantitative RT - PCR analysis of the de-greening circuit
genes (Figure 4E) corroborates the weak response observed in the transgenic plants, with
endogenous NtPOR mRNA levels decreasing to approximately one - third of the control levels, and AtRCCR increasing three-fold.
In the new study, a team led by Harvard University's George Church — one of the pioneers of the CRISPR technique — used
gene editing to remove all the copies of porcine
endogenous retroviruses (or PERVs) from their pig cells» DNA.
This conclusion is reinforced by the findings of Buller et al. (J: 8497), who showed that the Rmcfr allele contains an
endogenous MCF gp70 env
gene and that the Rmcfs allele, at least in some strains (C57BL / 6, CBA / J, and A / WySn), contains a xenotropic gp70 env
gene.
To accomplish this, we used homologous recombination to ablate nonmuscle myosin heavy chain (NMHC) II - B by inserting cDNA encoding green fluorescent protein (GFP)- NMHC II - A into the first coding exon of the Myh10
gene, thereby placing GFP - NMHC II - A under control of the
endogenous II - B promoter.
Bach1 has an
endogenous ligand, heme, that inhibits Bach1 binding to ARE, thus allowing Nrf2 - mediated
gene expression including that of heme - oxygenase - 1 (HMOX1), a well described target of Bach1 repression.
The use of
endogenous HMOX1 protein expression as a readout allowed the identification of compounds that specifically derepress Bach1 and induce transcription of an Nrf2 - responsive
gene.
In mouse models in which the
endogenous Smn1
gene has been knocked out and human versions of SMN2 have been swapped in, the Isis therapy — a so - called «antisense oligonucleotide» — delivered to the mouse central nervous system (CNS) increased the expression of full - length SMN protein in motor neurons, improved muscle strength in behavioral tests and extended the rodents» median lifespan from 16 days to 26 days3.
Recently, prospects for xenotransplantation brightened when Harvard geneticist George Church demonstrated the removal of dozens of
endogenous retroviruses from the pig genome, in a tour de force of the CRISPR / Cas9
gene editing technique.
Next, we repeated the transient transfection using NSCs derived from the H1 hESC line that contains the EGFP reporter cassette knocked in the
endogenous Oct4
gene by homologous recombinantion [35].
Here, we hypothesize that XJB -5-131 will lower IOP by decreasing
endogenous TGF - Î ² 2
gene expression and protein secretion.
In each of the BAC transgenic vectors,
endogenous protein coding sequences have been replaced by sequences encoding the EGFP reporter
gene.
In fact, NLuc is bright enough to detect
endogenous tagged
genes generated through CRISPR / Cas9 knock - in.
We applied an integrated proteomic and genetic strategy by targeting a tandem affinity purification (TAP) tag and Venus fluorescent protein into the
endogenous Arc
gene in mice.