Development of a real - time quantitative RT - PCR (Taqman) assay to
measure cytokine profiles in California sea lions (Zalophus californianus) and Bottlenose dolphins (Tursiops truncatus).
Not exact matches
The immune responses will be evaluated in the different species by
measuring influenza antigen - specific IgG / IgA and IgM, neutralising antibodies and antibodies inhibiting hemagglutination and antigen specific lymphocyte responses and
cytokine secretion
profiles by advanced flow cytometry.
This will include testing the mechanism of action of different adjuvant and antigen formulations by
measuring B - and T - cell responses and by using various functional assays (ELISA, FACS,
cytokine profiles), transcriptomics and / or proteomics.