Sentences with phrase «measured expression levels»

Gene expression patterns in the hippocampus samples were examined using DNA microarrays that measured the expression levels of over 30,000 genes (transcripts) per sample.

Quantitative PCR using the UPL system was carried out to measure the expression levels.

Not exact matches

During his time at the Pride Agenda he has been actively involved with the passage of dozens of laws, ordinances, regulations and Executive Orders on the state and local level affecting New York's LGBT community, including statewide measures like: the Sexual Orientation Non-Discrimination Act; the Hate Crimes Act of 2000; making the state's 9/11 relief inclusive of same - sex couples; guaranteeing domestic partners hospital visitation, legal authority over a loved one's bodily remains, access to Family Court and medical decision making authority; prohibiting discrimination on the basis of gender identity and expression in state employment; and securing over $ 50 million of funding for LGBT health and human services.

Then they compared the expression of thousands of genes in two samples by measuring levels of messenger RNA.

To investigate, they measured mRNA levels associated with the expression of 23,000 genes in human brain tissue.

Specifically, the researchers measured leukocyte (white blood cell) RNA expression levels.

In combination with a separate technique that measures the degree to which a gene is expressed, the researchers then identified genes that were either mutated or functionally altered through expression levels in these bladder cancer cell lines.

The SPFES is a tool to measure pain levels based on facial expressions of sheep, and has been shown to recognise pain with high accuracy.

Gene expression divergence levels were obtained from [71] and were measured in terms of the number of commonly co-expressed genes between human and mouse one to one orthologs.

We induced seizure by awakening the Japanese honeybee workers from CO2 - induced anesthesia at room temperature (25 °C) as described previously [13], and measured the Acks expression levels in the whole brain using quantitative RT - PCR.

The method, PrediXcan, estimates gene expression levels across the whole genome — a better measure of biological action than single mutations — and integrates it with genome - wide association study (GWAS) data.

Supplementary Fig. 36a shows the performance of the model based on the TIN measure at the tissue level, while Supplementary Fig. 36b compares the predictions based on TIN with the predictions based on gene expression at the individual level.

«Methods currently used for measuring gene expression generally rely on something called «relative normalisation», which means that gene expression levels in a sample can only be estimated relative to other genes in the same sample.

Dr Gilchrist said: «This study suggests that we may improve significantly on the widely used analysis methods for determining gene expression levels from high throughput sequence data: absolute quantitation offers a much sounder basis for determining changes in gene expression level, a measure widely used to determine the consequence of genetic, chemical or physical disturbances in living systems.»

To directly measure the level of coordination in the expression of genes, we used highly sensitive fluorescence in situ hybridization (FISH) to count individual mRNAs of functionally related and unrelated genes within single Saccharomyces cerevisiae cells.

mRNA molecules contain the instructions for making proteins — the number of mRNAs from a given gene is a measure of the level of expression of that gene.

Confirmation of hypoxic conditions was carried out using immunohistochemistry to measure expression of HIF - 1α and qRT - PCR to assess upregulation of target genes at the RNA level (Supplementary Fig.

To annotate the genome, the team generated transcriptome sequence data — which can be used to measure gene expression based on RNA levels — in 12 different tissues types.

Third, wild - type and toddler mutant embryos were injected at the one cell stage with increasing levels of Nodal mRNA and Nodal target gene expression was measured just prior to gastrulation using qRT - PCR.

The addition of sacha inchi oil slowed down glucose production in 16 (38.1 %) participants that showed a higher concentration of triglycerides before meals (fasting hypertriglyceridemia), whereas an improved expression of sirtuin - 1 (SIRT1), a gene responsible for regulating energy within the cells and coordinating cellular functions, was registered four hours after ingesting the meal enriched with sacha inchi oil, and linked to lowered levels of blood sugar measured at the same time.

Blood samples were also collected at the beginning and end to measure gene expression and levels of inflammation.

73 Moreover, as regards those resources, the expression «recourse to the social assistance system» in Article 7 (1)(c) of Directive 2003/86 does not allow a Member State to refuse family reunification to a sponsor who proves that he has stable and regular resources which are sufficient to maintain himself and the members of his family, but who, given the level of his resources, will nevertheless be entitled to claim special assistance in order to meet exceptional, individually determined, essential living costs or income support measures (see Chakroun, paragraph 52).

The current study measured adolescent perceptions of maternal anxious parenting (a combination of overprotection and expression of anxiety), mothers» levels of anxiousness, and adolescents» anxiety symptoms in 421 girls in grade 7 and their mothers.