Sentences with phrase «mediated gene knockout»

Not exact matches

Programmable nucleases, ZFN, TALEN and RGENs enable gene knockout in cultured cells and organisms by producing site - specific DNA double - strand breaks, whose repair via error - prone non-homologous end joining (NHEJ) or microhomology - mediated end joining (MMEJ) gives rise to frameshift mutations.

Here, we report that CRISPR / Cas9 mediated approach can efficiently induce monoallelic and biallelic gene knockout in goat primary fibroblasts.