Sentences with phrase «mediated gene targeting»

Cas9 / gRNA - mediated gene targeting demonstrated in this study can be used for other livestock species, which will contribute to advancing transgenic applications of large animal in biomedical and agricultural sciences.

Independent control of immunoglobulin switch recombination at individual switch regions evidenced through Cre — loxP — mediated gene targeting.

Not exact matches

The protein products of these genes, the authors note, «represent targets for immunotherapy, because inactivating mutations sensitize tumor cells to T - cell mediated attack.»

We are investigating the genes that are the target of this pathway, how it influences the cells of growing worms, the size differences between the sexes (hermaphrodite worms are larger than males), and the way in which it mediates signals from the environment.

I am interested in understanding how genetic polymorphisms in genes encoding PTPs can mediate disease pathogenesis, and in defining new drug targets for immune - mediated diseases among the PTPome.

We used RNA - mediated interference to target 98 % of all genes predicted in the C. elegans genome in combination with differential interference contrast time - lapse microscopy.

We design short interfering RNA (siRNA) molecules that are able to trigger the RNAi pathway and mediate the degradation of specific target messenger RNAs (mRNAs), resulting in the expression of a particular disease - causing gene being inhibited.

We have demonstrated that our liposomes can mediate in vivo target mRNA delivery as well as gene disruption using an entirely RNA - based approach.

Career Goals: My goal is to pursue a career in scientific research that focuses on identifying gene targets for treating / ameliorating immune - mediated inflammatory diseases.

These GEMM - ESCs, with often multiple modified alleles, form the basis for further genetic engineering either by Flp - recombinase mediated integration, gene targeting or Crispr / Cas9 to allow for the evaluation of altered target gene expression in a spontaneous tumor model.

Bach1 has an endogenous ligand, heme, that inhibits Bach1 binding to ARE, thus allowing Nrf2 - mediated gene expression including that of heme - oxygenase - 1 (HMOX1), a well described target of Bach1 repression.

However, we can not rule out the possibility that ABL kinases might regulate YAP1 - mediated expression of other target genes in breast cancer cells.

Cell - Specific Targeting Strategies for Electroporation - Mediated Gene Delivery in Cells and Animals.

We are particularly interested in how miRNAs can generate thresholds in target gene expression and mediate feedforward and feedback loops in gene networks.

Abbreviations: Aβ, amyloid β - peptide; AD, Alzheimer's disease; ALS, amyotrophic lateral sclerosis; Ambra1, activating molecule in Beclin -1-regulated autophagy; AMPK, AMP - activated protein kinase; APP, amyloid precursor protein; AR, androgen receptor; Atg, autophagy - related; AV, autophagic vacuole; Bcl, B - cell lymphoma; BH3, Bcl - 2 homology 3; CaMKKβ, Ca2 + - dependent protein kinase kinase β; CHMP2B, charged multivesicular body protein 2B; CMA, chaperone - mediated autophagy; 2 ′ 5 ′ ddA, 2 ′, 5 ′ - dideoxyadenosine; deptor, DEP - domain containing mTOR - interacting protein; DRPLA, dentatorubral pallidoluysian atrophy; 4E - BP1, translation initiation factor 4E - binding protein - 1; Epac, exchange protein directly activated by cAMP; ER, endoplasmic reticulum; ERK1 / 2, extracellular - signal - regulated kinase 1/2; ESCRT, endosomal sorting complex required for transport; FAD, familial AD; FDA, U.S. Food and Drug Administration; FIP200, focal adhesion kinase family - interacting protein of 200 kDa; FoxO3, forkhead box O3; FTD, frontotemporal dementia; FTD3, FTD linked to chromosome 3; GAP, GTPase - activating protein; GR, guanidine retinoid; GSK3, glycogen synthase kinase 3; HD, Huntington's disease; hiPSC, human induced pluripotent stem cell; hVps, mammalian vacuolar protein sorting homologue; IKK, inhibitor of nuclear factor κB kinase; IMPase, inositol monophosphatase; IP3R, Ins (1,4,5) P3 receptor; I1R, imidazoline - 1 receptor; JNK1, c - Jun N - terminal kinase 1; LC3, light chain 3; LD, Lafora disease; L - NAME, NG - nitro - L - arginine methyl ester; LRRK2, leucine - rich repeat kinase 2; MIPS, myo - inositol -1-phosphate synthase; mLST8, mammalian lethal with SEC13 protein 8; MND, motor neuron disease; mTOR, mammalian target of rapamycin; mTORC, mTOR complex; MVB, multivesicular body; NAC, N - acetylcysteine; NBR1, neighbour of BRCA1 gene 1; NOS, nitric oxide synthase; p70S6K, ribosomal protein S6 kinase - 1; PD, Parkinson's disease; PDK1, phosphoinositide - dependent kinase 1; PE, phosphatidylethanolamine; PI3K, phosphoinositide 3 - kinase; PI3KC1a, class Ia PI3K; PI3KC3, class III PI3K; PI3KK, PI3K - related protein kinase; PINK1, PTEN - induced kinase 1; PKA, protein kinase A; PLC, phospholipase C; polyQ, polyglutamine; PS, presenilin; PTEN, phosphatase and tensin homologue deleted from chromosome 10; Rag, Ras - related GTP - binding protein; raptor, regulatory - associated protein of mTOR; Rheb, Ras homologue enriched in brain; rictor, rapamycin - insensitive companion of mTOR; SBMA, spinobulbar muscular atrophy; SCA, spinocerebellar ataxia; SLC, solute carrier; SMER, small - molecule enhancer of rapamycin; SMIR, small - molecule inhibitor of rapamycin; SNARE, N - ethylmaleimide - sensitive factor - attachment protein receptor; SOD1, copper / zinc superoxide dismutase 1; TFEB, transcription factor EB; TOR, target of rapamycin; TSC, tuberous sclerosis complex; ULK1, UNC -51-like kinase 1; UVRAG, UV irradiation resistance - associated gene; VAMP, vesicle - associated membrane protein; v - ATPase, vacuolar H + - ATPase; Vps, vacuolar protein sorting

268/4: 45 Identifying the transcription factors mediating enhancer — target gene regulation in the human genome.

Furthermore, cold - mediated induction of genes related to thermogenesis such as Ucp1, Elovl3, Cox7a1, Cox8, and Cidea, as well as many PPAR target genes, was similar in wildtype and PPARα − / − mice.

We have generated new genetic tools for the analysis of in vivo mast cell functions that allow for cell type - specific inactivation («knock out «-RRB- of target genes selectively in mast cells by Cre loxP - mediated recombination.

1) Plant - mediated gene silencing: Using plant - mediated gene silencing, scientists induce plants to transfer pieces of genetic material to other organisms, targeting and interfering with the interactions between plants and their pests at the genetic level.

Both sets of findings suggest that preventive interventions that change parenting behavior may have differential effects on this proximal target depending on baseline levels of parenting, which can mediate earlier gene - environment interplay for both parent and child.