Lentiviral shRNA —
mediated knockdown of STAT5A and STAT5B was conducted following the manufacturer's instructions (Dharmacon RHS4531 - EG6776, EG6777).
Lentiviral shRNA —
mediated knockdown of ABL1 / ABL2 and expression of mouse Abl1 / Abl2 were conducted as described previously (20, 21, 59).
Not exact matches
The researchers were surprised to find that
knockdown of one specific mitochondrial chaperone, mtHSP70, elicited a unique stress response
mediated by fat accumulation, resulting in improved protein folding in the interior or cytosol of the cell.
In order to amplify the efficiency of shRNA -
mediated gene
knockdown, UAS - dicer1 was constructed.
While siRNA
knockdown of Nrf2 markedly decreased steady - state levels of Nrf2 protein, siRNA
knockdown of Keap1 increased steady - state levels of Nrf2 protein as lack of Keap1 -
mediated degradation results in accumulation of Nrf2.
Acute
knockdown of either protein using shRNAs resulted in an approximately 50 % reduction in AMPA receptor -
mediated EPSCs, and ~ 75 % loss if both proteins were knocked out simultaneously.
Further studies showed that Notch1
knockdown in NSCLC helps induce cell death (which inhibits tumor initiation and growth); this process is
mediated by p53, and thus the effects of inhibiting Notch1 require functional p53.