To obtain a viable donor of a Descemet
membrane endothelium disc, we modified the Descemet membrane stripping technique and monitored the percentage of endothelial damage to the donor tissue preparation.
Not exact matches
Using the TEER - MEA chip, the researchers built a beating vascularized Heart Chip in which human cardiomyocytes are cultured in one microfluidic channel that is separated by a thin semi-permeable
membrane from a second, parallel
endothelium - lined vascular channel.
In drug development, particular attention is paid to the expression and function of
membrane transporters expressed in epithelia of the intestine, liver and kidney, and in the
endothelium of the blood - brain and blood - testis barriers.
Multiple hematoxylin - eosin - stained sections showed the presence of
endothelium with intact Descemet
membrane and no stromal tissue.
A 6 × 7 - mm rectangular piece of
endothelium - Descemet
membrane complex was obtained using a Grieshaber microsurgical knife and Kelman - McPherson forceps.
Conclusion: We modified the technique of Melles and obtained a sheet of Descemet
membrane and
endothelium with minimal endothelial damage and with no remaining stroma observed.
Purpose: Replacing diseased corneal
endothelium with a preparation of Descemet
membrane carrying functional
endothelium and no stroma may be a feasible method for treating corneal endothelial decompensation.
Results: Forty of 48
endothelium - Descemet
membrane preparations (83.3 %) were complete peels with minimal endothelial damage.
Here we present a novel human Airway Lung - Chip containing a fully - differentiated human mucociliary airway epithelium separated from a pulmonary microvascular
endothelium by a semi-permeable
membrane that allows for immune cell infiltration.
The primary epithelial cells are expanded as 3D organoids, dissociated, and cultured on a porous
membrane within a microfluidic device with human intestinal microvascular
endothelium cultured in a parallel microchannel under flow and cyclic deformation.