Sentences with phrase «mirna microarray analysis»

Other labs have used DNA microarray analysis to characterize gene expression changes in this model.

However, microarray analysis is only capable of measuring the status of known transcripts, and expression of low - abundance mRNAs is often not detected by the hybridization - based approach.

Eager to sort through the pile of curriculum vitae's on his desk, Rudnicki is particularly looking for molecular geneticists working on different stem cell systems; scientists with genomics bents like proteomics, microarray analysis, and bioinformatics; and a couple of clinician scientists.

The foil - based measuring chip uses the microarray analysis platform MCR of the Munich company GWK GmbH.

The researchers then used microarray analysis and pre-clinical animal assays to identify two predominant glioblastoma subtypes, proneural and mesenchymal.

Apart from science and engineering, bioinformatics is a vital tool in microarray analysis due to the vast amounts of data that are generated from a microarray experiment.

To help identify genes and signaling pathways that may be influenced by zinc, Knoell and his team performed a genome - wide microarray analysis of lung tissue taken from zinc - deficient mice with sepsis.

Microbial pathogens induce specific host responses or «RNA biosignatures» that can be identified using microarray analyses of blood leukocytes (white cells).

A deep global analysis of the gut microbiota composition was done by phylogenetic microarray analysis using a Human Intestinal Tract Chip (HITChip), an analytical device designed specifically for studying gut bacteria.

Stephen W. Scherer, Ph.D., of the Hospital for Sick Children, Toronto, and colleagues performed chromosomal microarray analysis (CMA) and whole - exome sequencing (WES) in a group of 258 unrelated children with ASD to determine the molecular diagnostic yield (the percentage of subjects with a genetic alteration [mutation] that may contribute to the features of autism spectrum disorder) of these tests.

«We tried to do a thorough study,» Scherer says, «so that we could convince people to do a microarray analysis as part of their workup.»

(A) Location of the eye field (EF), PNP, and LE tissue isolated from stage 15 Xenopus embryos for microarray analysis.

Microarray analysis was performed at different time points to determine yeast genome - wide response when exposed to salt, acid, hypoxic conditions, and hydrogen peroxide, as well as ethanol, isopentenol, and other biofuels candidates.

Antibody microarray analysis holds promise for increasing accuracy of breast cancer screening

Therefore, we used microarray analysis to perform pairwise comparisons of the transcriptional profile of EFTF - expressing pluripotent cells to three regions of the Xenopus embryo: the eye field (EF) including underlying mesendoderm, posterior neural plate (PNP) and the non-neural, flank, lateral endoderm (LE, Figure 1A).

This will be achieved by exposing the coral to seawater with high calcium concentrations to induce an alteration in gene expression profiles, identified using a DNA microarray analysis.

Parallel whole - genome microarray analysis was used to determine the transcriptome in matched samples.

Yet, pericytes themselves have been shown to be a primary source of Ang1 (refs 21, 22, 23)(which was confirmed in the microarray analysis (Fig. 1a)-RRB-, suggesting an autocrine mode of action.

The names of samples within the microarray analysis were labeled C11 and T11 as for control (reference) and treatment at daytime (11:00), respectively.

Along with the cellular genes emphasized from the microarray analysis, two genes that are suspected to be involved in the scleractinian coral calcification process were tested for expression (Figs. 5A and 5B).

Microarray Analysis: Anti-Human SMAD4 (clone R516.2.1 D12) specificity was evaluated using CDI HuProt ™ Human Proteome Microarray and subsequently analyzed with GenePix Pro Image Acquisition and Analysis Software, the benchmark tool for the acquisition and analysis of microarray images.

Microarray analysis revealed temperature - dependent expression of distinct sets of genes, as well as 700 A. fumigatus genes not present or significantly diverged in the closely related sexual species Neosartorya fischeri, many of which may have roles in the pathogenicity phenotype.

Microarray Analysis: Test antibody specificity was evaulated using CDI HuProt ™ Human Proteome Microarray and subsequently analyzed with GenePix Pro Image Acquisition and Analysis Software, the benchmark tool for the acquisition and analysis of microarray images.

Microarray Analysis: Test antibody specificity was evaulated using CDI HuProt ™ Human Proteome Microarray (~ 75 % of the human proteome) and subseqently analyzed with GenePix Pro Image Acquisition and Analysis Software, the benchmark tool for the acquisition and analysis of microarray images.

Under the stewardship of Christine Armour, the working group, consisting of members from the CCMG Clinical Practice and Laboratory Practice committees with representatives from the SOGC, have updated the 2001 Canadian guidelines for use of chromosomal microarray analysis (CMA).

Proteomic analysis to profile protein abundance resulted in the identification and relative quantification for 912 proteins with two or more unique peptides and 86 proteins with significant abundance changes after treatment with the neurotoxins, while microarray analyses to profile gene expression revealed 181 genes with significant changes in mRNA after treatment.

Application of microarray analysis to the investigation of host - pathogen interactions.

Microarray Analysis: Test antibody specificity was evaulated using CDI HuProt ™ Human Proteome Microarray (19,394 human proteins) and subseqently analyzed with GenePix Pro Image Acquisition and Analysis Software, the benchmark tool for the acquisition and analysis of microarray images.

Demonstration of loss of heterozygosity by single - nucleotide polymorphism microarray analysis and alterations in strain morphology in Candida albicans strains during infection.

Finally, the researchers used microarray analysis to investigate the gene expression and molecular pathways in the muscles of DS mice.

Microarray Analysis Confirms the Specificity of a Chlamydomonas reinhardtii Chloroplast RNA Stability Mutant

We also performed microarray analysis of PyMT - Slc6a14 + / + and PyMT - Slc6a14 − / − mammary tumours to evaluate the expression of amino acid transporters without any biased preconception of the identity of the transporters that might be subject to changes in expression in association with Slc6a14 deletion.

For some applications, a qPCR wil... Keywords: Amplification, Cancer, Capillary electrophoresis, Cell culture, Degradations, Detection methods, Electrophoresis, Gene expression, Growth factors, Indicators, Microarray Analysis, Polymerase chain reaction, Polymerase chain reaction - quantitative, Purification, RNA purification, Sample preparations, Transcription

Microarray analysis of prostate cancer progression to reduced androgen dependence: studies in unique models contrasts early and late molecular events.

Biotin - l... Keywords: Arylations, Cross couplings, DNA replication, Diagnostic, Gene expression, Microarray Analysis, Polymerase chain reaction, Polymerase chain reaction - quantitative, Sequencing

The summary below highlights the applications of commonly used custo... Keywords: Extinction coefficient, Gene expression, High performance liquid chromatography, Microarray Analysis, Polymerase chain reaction - quantitative

Keywords: Chromatography, Cloning, Electrophoresis, Filtration, Gas chromatography, Gel electrophoresis, High performance liquid chromatography, Microarray Analysis, Nuclear magnetic resonance spectroscopy, PAGE, Polymerase chain reaction, Polymerase chain reaction - quantitative, Purification, Reversed - phase chromatography, Separation, Sequencing, Solvents

Recently, Dr. Wapner led a multicenter study evaluating the accuracy, efficacy, and clinical advantages of prenatal diagnosis using microarray analysis.

Keywords: Gene expression, High performance liquid chromatography, Mass spectrometry, Microarray Analysis, Nucleic acid hybridization, Polymerase chain reaction - quantitative, Purification

They employed whole genome microarray analysis to perform sample - matched transcriptome analyses.

Similarly, microarray analyses comparing global gene expression in cells that overexpress wild - type STAT3 or phosphorylation - deficient mutant indicate that a subset of genes are regulated by unphosphorylated STAT3, including well - known oncogenes such as MRAS and MET (Yang et al., 2005).

Whole - genome sequencing, chromosomal microarray analysis, and pharmacogenomics present new ethical questions and dilemmas for physicians to consider.

Comparison of gene expression profiles obtained by Q - PCR (left panels) and microarray analysis (right panels).

41) Chari R, Lockwood WW, Coe BP, Chu A, Macey D, Thomson A, Davies JJ, MacAulay C, Lam WL (2006) SIGMA: a system for integrative genomic microarray analysis of cancer genomes.

Finally, RNA samples were prepared following the same procedure and subsequent microarray analyses were performed in a central facility using Affymetrix Mouse 430 v. 2 arrays.

Microarray analysis, the researchers found, revealed genetic conditions in 2.5 % of the pregnancies with a normal karyotype.

Microarray Analysis of Retinal Gene Expression in the DBA / 2J Model of Glaucoma Steele, MR., Inman DM., Calkins, D.J., Horner, P.J. & Vetter, M.L. (2006) Investigative Ophthalmology & Visual Science, 47:977 - 985.

264/5: 45 Diagnostic utility of whole genome sequencing as an alternative to chromosomal microarray analysis in pediatric medicine.

Antibody Cross-Reactivity Testing Using the HuProt ™ Human Proteome Microarray Analysis of several commercially available antibodies against nearly 75 % of the human proteome.

After microarray analysis, available remaining material corresponding to 17 total and / or polyA RNA samples (13 unique samples) were tested for XMRV by gag RT - PCR.