Sentences with phrase «microarray assay»

The discovery of XMRV was accomplished using a broad - spectrum microarray assay (ViroChip) designed to detect all known viruses as well as novel viruses on the basis of sequence homology [1], [2], [3].

It is a paper based vertical flow microarray assay that could find use in future point of care affinity proteomic applications, for instance in the fields of autoimmunity, allergy, infection or cancer diagnostics.

DS carried out cell culture experiments and JLE performed DNA microarray assay and corresponding data analysis.

In this study, we employed a pan-viral microarray assay, the Virochip, to identify a novel adenovirus associated with a fulminant pneumonia outbreak in a colony of New World titi monkeys.

Not exact matches

The researchers then used microarray analysis and pre-clinical animal assays to identify two predominant glioblastoma subtypes, proneural and mesenchymal.

Furthermore, they are undertaking an additional validation measure on several SNPs via the TaqMan ® assay, a non — microarray - based genotyping method.

To gain a deeper understanding of the cellular response of the two neurotoxins, the researchers applied global quantitative proteomics at the U.S. Department of Energy's national user facility — the Environmental Molecular Sciences Laboratory — and microarrays at UCLA to assay the protein and gene levels, respectively, for both neurotoxins.

Quantitative real time PCR (qRT - PCR) is accepted as the gold standard for expression profiling, so we next compared both our RNAseq and the microarray expression estimates to a panel of qRT - PCR TaqMan gene expression assays (Figure 2C — F).

The microarray allows the specificity of fluorescently labeled, biotinylated or radiolabeled molecules to be assayed against the largest collection of human proteins in the world.

(C — F) For a subset of genes (Table S1), the log10 normalized intensity values obtained from the microarrays (C — D) and from RNAseq in FPKM (E — F) are plotted against the log10 RQ values from TaqMan qRT - PCR assays.

XMRV was not detected in a new set of 39 prospectively collected prostate tumors (both with or without RNAse L mutations) by PCR assays performed independently in 3 different laboratories or ViroChip microarray.

«The microarrays allowed us to assay every single gene that was in the original sequence strain for its presence or absence in the other strains,» says Israel.

Combining our in vitro assay with microarrays analyses, we have uncovered previously unknown effectors and target genes of Wnt.