The main objective is to generate RNA that is of high quality and sufficiently concentrated for use in
a microarray experiment.
GeneTraffic is a bioinformatics server system for microarray data storage and analysis in Minimum Information about
a Microarray Experiment (MIAME) compliant format [50].
Apart from science and engineering, bioinformatics is a vital tool in microarray analysis due to the vast amounts of data that are generated from
a microarray experiment.
In
the microarray experiment, the range in relative abundances of the 500 most highly expressed genes is very narrow (Figure 1 inset), especially in contrast to RNA - Seq technology (Figure 1).
We also found that RNA sequencing yields superior results compared to
microarray experiments.
Guidelines exist for the appropriate design and conduct of studies in some fields, including animal research and
microarray experiments.
For instance, the National Center for Biotechnology Information's public repository of gene - expression data, GEO, now contains 392,000
microarray experiments.
Our microarray experiments had identified strong FOXO3A and weak FOXO1 expression in pericytes (Fig. 1a).
Interestingly,
the microarray experiments pursued in the present study had identified low levels of Tie2 expression in all analysed pericyte populations.
Unfortunately, direct comparisons of gene expression profiles obtained in independent, publicly available
microarray experiments are typically compromised by substantial, experiment - specific biases.
Study of stem cell function using
microarray experiments.
Generally,
microarray experiments can be completed within one week of receipt of a small number of samples.
Breeden, Prentice and Zhao, whose study appeared in the May 8 Proceedings of the National Academy of Sciences, compared their new algorithm to other methods used to analyze
microarray experiments designed to identify cell cycle genes in yeast.
Other activities (e.g. to provide recent information about recombinant DNA experiments, to support DNA / protein sequencing and
microarray experiments, and so on..)
However, the results of the Ago IP /
microarray experiments revealed that APC mRNA was much less efficiently recruited to RISC by miR - 150 (Figure 1A — source data 3 — 4).
For HEEBO
microarray experiments, poly - adenylated RNAs were amplified in the presence of aminoallyl - UTP with Amino Allyl MessageAmp II aRNA kit (Ambion).
Not exact matches
The goal of the first
experiment was to see whether lncRNAs are differentially expressed in prostate cancer by measuring total RNA from prostate cancer cell lines and normal epithelial prostatic cells using NCode human ncRNA array and SurePrint G3 human lncRNA
microarrays.
To produce standard protein
microarrays small amounts of proteins are printed to a glass slide and chemically fixed in each spot where they are then available for
experiments.
Protein
microarrays allow the investigation of thousands of proteins in a single
experiment.
Contact Mark Bedford (
[email protected]) for
experiments involving the use of protein domain
microarrays, and contact Shawn Bratton (
[email protected]) for
experiments involving the biophysical analysis of protein complexes.
The facility collaborates with various research projects using flow cytometric techniques to isolate and purify cells and chromosomes for structural and functional studies, sequencing,
microarrays as well as fluorescence insitu hybridisation (FISH)
experiment.
Affymetrix Expression Console Software (version 1.0) was used to perform quality assessment of the
microarray scan /
experiments.
DS carried out cell culture
experiments and JLE performed DNA
microarray assay and corresponding data analysis.
The challenge facing biologists now, say many Hutch scientists, is the need for new statistical models to extract useful information - a need that has not kept pace with the overwhelming number of
experiments conducted using
microarray technology.
Microarray data from an
experiment looking at oral - cancer development prompts a conversation in the Public Health Sciences lab of Dr. Chu Chen by (from left) Drs. Lue Ping Zhao, PHS investigator; Chun Cheng, PHS postdoctoral fellow; Chen and Eduardo Mendez of the University of Washington Department of Otolaryngology - Head and Neck Surgery.
Microarray (LS.1 j; LS.2 d; LS.13 a, f; BIO.5 f, i, j) Prior to the
experiment, students are navigated through a user guided animation on genomics and gene expression.