The aim of his research was to use
microarray gene expression data in order to characterize ageing in different human tissues and identify the age at which major changes in genetic expression profiles occur.
Not exact matches
In contrast, when the OncoFinder algorithm is applied to the
data, a clear correlation between next generation sequencing and
microarray gene expression datasets was seen.
Microarrays allow researchers to acquire
data about
expression levels of thousands of
genes at one fell swoop.
For instance, the National Center for Biotechnology Information's public repository of
gene -
expression data, GEO, now contains 392,000
microarray experiments.
The accumulation of adipose tissue macrophages in direct proportion to adipocyte size and body mass may explain the coordinated increase in
expression of
genes encoding macrophage markers observed in our
microarray expression data.
Using quantitative RT - PCR we confirmed the
expression profile of five
genes (colony - stimulating factor 1 receptor [Csf1r], Cd68, Pex11a, Emr1, and Mcp1) in each of the 24 samples and found excellent agreement between the
microarray and RT - PCR
expression data (mean Pearson correlation coefficient = 0.91,
microarray versus RT - PCR
expression; Supplemental Table 3, http://www.jci.org/cgi/content/full/112/12/1796/DC1).
These are among the
genes whose
expression in our
microarray expression data set correlated positively with body mass and adipocyte size.
For
expression analysis in mice, we used
microarray data as described above to select two internal control
genes, cyclophilin B (Cphn2) and ribosomal protein S3 (Rps3).
Gene Set Enrichment Analysis (GSEA) is a
microarray data - mining technique used to determine whether there is coordinated differential
expression or «enrichment» in a set of functionally related
genes when comparing control and experimental samples [35].
The raw
data as well as the processed
data of the
microarray were deposited under
Gene Expression Omnibus (GEO)(http://www.ncbi.nlm.nih.gov/geo) accession number GSE87159.
All
microarray data supporting the findings of this study have been deposited in the National Center for Biotechnology Information
Gene Expression Omnibus (GEO) and are accessible through the GEO Series accession number GSE86885.
Most of these compare
gene expression data (
microarray or RNA - seq) to high - density SNP array genotypes.
The
microarray data (
Gene Expression Omnibus accession No.
Expression of VR and OR genes in RNAseq compared with microarray expres
Expression of VR and OR
genes in RNAseq compared with
microarray expressionexpression data.
In brief, 330 of 364
genes, tested by quantitative or conventional PCR, gave comparable
expression patterns to the
data obtained by
microarray analysis.
Specifically, we have generated clusters of transcripts that behave the same way under the entire spectrum of the sixty - seven experimental conditions; we have assembled
genes in groups according to their time of
expression during successive days of ES cell differentiation; we have included expression profiles of specific gene classes such as transcription regulatory factors and Expressed Sequence Tags; transcripts have been arranged in «Expression Waves» and juxtaposed to genes with opposite or complementary expression patterns; we have designed search engines to display the expression profile of any transcript during ES cell differentiation; gene expression data have been organized in animated graphs of KEGG signaling and metabolic pathways; and finally, we have incorporated advanced functional annotations for individual genes or gene clusters of interest and links to microarray and genomic
expression during successive days of ES cell differentiation; we have included
expression profiles of specific gene classes such as transcription regulatory factors and Expressed Sequence Tags; transcripts have been arranged in «Expression Waves» and juxtaposed to genes with opposite or complementary expression patterns; we have designed search engines to display the expression profile of any transcript during ES cell differentiation; gene expression data have been organized in animated graphs of KEGG signaling and metabolic pathways; and finally, we have incorporated advanced functional annotations for individual genes or gene clusters of interest and links to microarray and genomic
expression profiles of specific
gene classes such as transcription regulatory factors and Expressed Sequence Tags; transcripts have been arranged in «
Expression Waves» and juxtaposed to genes with opposite or complementary expression patterns; we have designed search engines to display the expression profile of any transcript during ES cell differentiation; gene expression data have been organized in animated graphs of KEGG signaling and metabolic pathways; and finally, we have incorporated advanced functional annotations for individual genes or gene clusters of interest and links to microarray and genomic
Expression Waves» and juxtaposed to
genes with opposite or complementary
expression patterns; we have designed search engines to display the expression profile of any transcript during ES cell differentiation; gene expression data have been organized in animated graphs of KEGG signaling and metabolic pathways; and finally, we have incorporated advanced functional annotations for individual genes or gene clusters of interest and links to microarray and genomic
expression patterns; we have designed search engines to display the
expression profile of any transcript during ES cell differentiation; gene expression data have been organized in animated graphs of KEGG signaling and metabolic pathways; and finally, we have incorporated advanced functional annotations for individual genes or gene clusters of interest and links to microarray and genomic
expression profile of any transcript during ES cell differentiation;
gene expression data have been organized in animated graphs of KEGG signaling and metabolic pathways; and finally, we have incorporated advanced functional annotations for individual genes or gene clusters of interest and links to microarray and genomic
expression data have been organized in animated graphs of KEGG signaling and metabolic pathways; and finally, we have incorporated advanced functional annotations for individual
genes or
gene clusters of interest and links to
microarray and genomic resources.
The results show comparable
gene expression profiles between
microarray and Q - PCR
data.