Since amiodarone HCL is known to exert its cytotoxic effect through the extrinsic, caspase - 9 independent apoptotic pathway [33]
our microarray results confirm that the differential cytotoxic effect seen in NSCs treated with amiodarone HCL is due to specific activation of extrinsic apoptosis pathways resulting from exposure to the drug.
In addition, analysis of
the microarray results obtained with RNA from the optimized protocol showed that, as expected, the genes involved in methane production (methanogenesis) were among the most highly expressed genes in M. barkeri.
Not exact matches
Natera uses a SNP
microarray from Ilumina with a proprietary technology called Parental Support ™, which tests all 24 chromosomes and delivers
results that are typically > 99 % accurate.
We also found that RNA sequencing yields superior
results compared to
microarray experiments.
Last July, Perls and Sebastiani told Science that they were unaware of problems in the
microarrays and were working hard to verify their
results.
As ICREA Prof. at ICFO Valerio Pruneri comments «the device means a major step forward for light microscopy techniques, especially for
microarray platforms since it could definitely be used as a point - of - care tool in the diagnosis and treatment of major diseases such as Sepsis, a critical area where fast and accurate
results can translate into life changing health outcomes for individuals.
Gene
microarrays, which measure expression of dozens of genes at once, can be fickle and supply inconsistent
results.
The study
results challenge the current paradigm of
microarray data analysis and suggest that the new method may improve identification of cancer - associated genes.
Results: Benefiting from the recently sequenced genome of the model sulfate - reducing bacterium (SRB), Desulfovibrio vulgaris, microbiologist Weiwen Zhang and a team of scientists at Pacific Northwest National Laboratory (PNNL) are applying a suite of post-genomics technologies, including
microarray, proteomic, and computational analyses, to obtain a broader understanding of D. vulgaris» metabolism.
The
results, which will appear in the Journal of Microbiological Methods, make it clear that the methods used to isolate RNA can have a significant impact on the variability, trend, and accuracy of
microarray data.
Proteomic analysis to profile protein abundance
resulted in the identification and relative quantification for 912 proteins with two or more unique peptides and 86 proteins with significant abundance changes after treatment with the neurotoxins, while
microarray analyses to profile gene expression revealed 181 genes with significant changes in mRNA after treatment.
The reduction of Kap1 by IVC agrees with previous
results of
microarrays, wherein using a list of murine IAP elements, it was observed that several of the probes analyzed were intensely up - regulated in IVC [7].
Researchers performed ES cell cultures, RNA preparations and validated
microarray data: IA KA NB PYB MXD MG EK SL MR AR MPS MT.
Results were discussed among all Consortium partners.
We assessed the ability of a commercial DNA
microarray to characterize bovine Shiga toxin - producing Escherichia coli (STEC) isolates and evaluated the
results using in silico hybridization of the
microarray probes within whole genome sequencing scaffolds.
The
results show comparable gene expression profiles between
microarray and Q - PCR data.
Microarray analysis yielded
results in 87 % of the stillbirths, compared to 70 % for karyotype analysis.
However, the
results of the Ago IP /
microarray experiments revealed that APC mRNA was much less efficiently recruited to RISC by miR - 150 (Figure 1A — source data 3 — 4).