Individual 45 - mer oligonucleotides corresponding to mRNAs of each gene were used as probes, and immobilized on
microarray slides, as described below.
RNA samples were studied in triplicate (i.e., 3
microarray slides for each retina).
Not exact matches
DNA
microarrays consist of thousands of spots contained on a single 1 x 3 inch glass
slide, each spot representing a different gene.
Traditionally, gene function was determined one gene at a time, but today's
microarray technology enables scientists to monitor the expression of tens of thousands of genes on a single glass
slide.
To produce standard protein
microarrays small amounts of proteins are printed to a glass
slide and chemically fixed in each spot where they are then available for experiments.
Total RNA was labeled and hybridized against custom S. pistillata
microarray; an Agilent two - color gene expression
microarray platform with 8 × 15 K probe per
slide;
microarray samples included four SW +100 and four SW (control) samples for each time point (11:00 and 23:00).
The CDI HuProt
microarray contains nearly 75 % of the human proteome on a single glass
slide, a unique and powerful tool for high - throughput serum profiling and biomarker discovery.