Sentences with phrase «microarray studies»

Microarray studies have the potential to piece together groups of co-regulated genes and thus lead to the discovery of novel components of genetic pathways in ES cells.

Microarray studies and, more recently, exome sequencing have demonstrated the importance of de novo copy number variations (CNVs) and single - nucleotide variations (SNVs) in ID, but the majority of cases remains undiagnosed.

The expression rates of three macrophage - specific genes (Emr1, Cd68, and Csf1) that correlated with body mass in our microarray studies, an adipocyte - specific gene (Acrp30), and proinflammatory genes (Tnfa, Nos2, Il6) were determined by quantitative RT - PCR.

A microarray study of post-mortem mRNA degradation in mouse brain tissue.

Recently, a large - scale microarray study characterized gene expression differences between current, former, and never smokers [5], and identified specific genes related to xenobiotic functions, anti-oxidation, cell adhesion and electron transport to be more highly expressed in current smokers relative to never smokers.

Ultimately, the microarray study could provide clues about which genes are responsible for the disease and lead to the development of blood tests for early detection and possible new treatments for DCM.

Not exact matches

Whether you are studying the expression of thousands of genes simultaneously using DNA microarrays, or the interaction of multiple molecules to understand intercellular signalling, the skills that will help you understand your findings are statistics, computational techniques, and modelling — so all you really need is a mathematician.

This study, published in the journal Microarrays, shows that lack of SOST in the bone microenvironment promotes the expression of many genes associated with cell migration and / or invasion, including long non-coding RNA MALAT1 in prostate cancer, suggesting that SOST has an inhibitory effect on prostate cancer invasion.

On a study funded by the National Science Foundation and Agilent Technologies, researchers employed spatial light interference microscopy (SLIM), a label - free method, to perform localized measurements of light scattering in prostatectomy tissue microarrays.

Large - scale methods of probing samples, such as DNA sequencing, microarrays, and automated gene - function studies, are filling new databases to the brim.

When Mikovits used the microarray to study CFS patients, she «saw all these infections — lots of chronic, active infections — cytomegalovirus, Epstein - Barr virus, human herpes six,» she says.

Guidelines exist for the appropriate design and conduct of studies in some fields, including animal research and microarray experiments.

To study the variations, Chess and his colleagues turned to DNA microarrays.

Lead authors of the study, Ivan P. Gorlov, Ph.D., Associate Professor of Community and Family Medicine and Christopher Amos, Ph.D., Professor of Community and Family Medicine and Director of the Center for Genomic Medicine described a new method to analyze microarray data.

The study results challenge the current paradigm of microarray data analysis and suggest that the new method may improve identification of cancer - associated genes.

A deep global analysis of the gut microbiota composition was done by phylogenetic microarray analysis using a Human Intestinal Tract Chip (HITChip), an analytical device designed specifically for studying gut bacteria.

Then they studied the mice's lungs microscopically and, using microarrays, measured the expression of many dozens of genes in their lung tissue at 1, 3, and 5 days after infection.

«We tried to do a thorough study,» Scherer says, «so that we could convince people to do a microarray analysis as part of their workup.»

A total of 24 mice were included in the microarray expression study, four from each of the following groups: C57BL / 6J males, C57BL / 6J females, high fat — fed C57BL / 6J males, B6.Cg Ay / + females, B6.V Lepob / ob males, and B6.V Lepob / ob females.

Periostin (POSTN) was identified by microarray RNA profiling studies as a stromal factor involved in maintaining the normal stem cell niche and demonstrated to be secreted by stromal fibroblasts, but not by infiltrating tumor cells (Malanchi et al., 2012).

In this study, we employed a pan-viral microarray assay, the Virochip, to identify a novel adenovirus associated with a fulminant pneumonia outbreak in a colony of New World titi monkeys.

Related research (Pereira HJ, et al., 2012) used two different lines of evidence to explain the increase in the ratio of anteiso — to iso - branched alkenes that was observed during the transition from early to late stationary phase in M. luteus: structural studies of a key enzyme involved in fatty acid biosynthesis in M. luteus (FabH, or ß - ketoacyl - ACP synthase III) and transcriptional (whole - genome microarray) studies of M. luteus during different growth phases.

RNA samples were studied in triplicate (i.e., 3 microarray slides for each retina).

The European study was called MINDACT for Microarray for Node - Negative Disease May Avoid Chemotherapy Trial.

[NEWS, 4 June 2013] Protein microarrays, representing more than one third of all human proteins, provide a unique possibility to study complex autoimmune diseases, such as multiple sclerosis (MS).

In the present study, we therefore used microarray technology to investigate the concurrent expression of 1,178 genes in the rat retina both following hypoxia and following a post-hypoxic 24 h reoxygenation period.

The facility collaborates with various research projects using flow cytometric techniques to isolate and purify cells and chromosomes for structural and functional studies, sequencing, microarrays as well as fluorescence insitu hybridisation (FISH) experiment.

All microarray data supporting the findings of this study have been deposited in the National Center for Biotechnology Information Gene Expression Omnibus (GEO) and are accessible through the GEO Series accession number GSE86885.

Interestingly, the microarray experiments pursued in the present study had identified low levels of Tie2 expression in all analysed pericyte populations.

Here we suggest a novel yet conceptually simple approach for deriving «Functional Association (s) by Response Overlap» (FARO) between microarray gene expression studies.

Study of stem cell function using microarray experiments.

The microarrays used in the study were not confined to specific genes only but spanned the entire genome.

Microarray analysis of prostate cancer progression to reduced androgen dependence: studies in unique models contrasts early and late molecular events.

Recently, Dr. Wapner led a multicenter study evaluating the accuracy, efficacy, and clinical advantages of prenatal diagnosis using microarray analysis.

The students are employing that technology — which includes CRISPR (clustered regularly interspaced short palindromic repeats) systems, DNA microarrays, real - time polymerase chain reactions, RNA sequencing and western blotting — to study acute myeloid leukemia (AML), which affects both children and adults and comes with a high mortality rate for older patients.

In the present study we have reported the transcriptional profile of the two main components of the olfactory system in mice, obtained by RNAseq and expression microarray.

Services include phlebotomy, tissue microarrays, collection of pathological specimens and support for clinical studies.

The current study included 3,215 RS participants who had both SNP microarray data and 3D MRI.

Archival prostate tissue corresponding to the previously XMRV - positive VP62 sample (QQ) and derived from the same tissue block used in the original 2006 Urisman et al. study [1], further referred to as VP62 (2012), was also available for microarray screening.

Advances in chromosome microarray (CMA) testing narrowed the 3p25 critical region to a 124 kb region, and recent Whole Exome Sequencing (WES) studies have suggested that the SETD5 gene contributes significantly to the 3p25 phenotype.

This tool also allows conversion of previous Affymetrix probe set numbers (i.e., the first generation of Affymetrix microarrays - U74v2) to the more recent microarray probe set numbers (like the MG430v2 used in this study).

In this study, we investigated the putative association between XMRV and prostate cancer using a combination of microarray, PCR, FISH, serological, and deep sequencing approaches.

The study, «Preferential Recognition of Avian - Like Receptors in Human Influenza A H7N9 Viruses,» received support from the National Institutes for Health (R56 AI099275), the Skaggs Institute for Chemical Biology, the Scripps Microarray Core Facility, the Centers for Disease Control and the Netherlands Organization for Scientific Research.

Breeden, Prentice and Zhao, whose study appeared in the May 8 Proceedings of the National Academy of Sciences, compared their new algorithm to other methods used to analyze microarray experiments designed to identify cell cycle genes in yeast.

The SMF's microarray service offers a comprehensive set of integrated gene expression services that facilitate studying the role of genomic alterations and transcriptional regulation of genes.

All ViroChip microarrays used in this study have been deposited in the NCBI GEO database (accession numbers GSE39684 and GSM977688 - GSM977750).

Biologists are eager to use microarrays for such studies, Breeden said, but current analytical tools don't provide enough information to be useful for taking the next step in the lab.

Prior to working at City of Hope, she worked as a research associate in Dr. Peter Lee's lab at Stanford University since 2006 and facilitated in investigating mechanisms causing immune dysfunction in melanoma and breast cancer patients» peripheral blood, lymph node and tumor specimens utilizing techniques including microarray analysis, RT - PCR, Phospho - flow cytometry, histoloogy, and functional FACS studies.

The aim of the Deciphering Developmental Disorders (DDD) Study is to advance clinical genetic practice for children with developmental disorders by the systematic application of the latest microarray and sequencing methods while addressing the new ethical challenges raised.

«Low - Calorie Diet Slows Aging in Mice in Study,» claimed a recent headline.17 According to the article, «Putting elderly mice on a very low - calorie diet for as little as four weeks reversed many of the changes in the activity of various genes that had occurred during normal aging...» The resesearchers were not looking at actual signs of disease, nor were they measuring lifespan, but instead focused on the analysis of 11,000 different genes using a method called microarray technology in which Spindler has large financial holdings.