Sentences with phrase «microarrays hybridization»

A detailed description of the methods and analyses of methylated DNA immunoprecipitation (MeDIP) and microarrays hybridization used in this study were previously described [43] and can be found in File S1.

Target preparation and microarray hybridization — Total RNA was extracted from retinal samples using RNeasy Lipid Tissue Mini Kit (Qiagen) and was the substrate for amplification and labeling using a procedure based on the Eberwine protocol [49].

Microarray hybridization patterns were interpreted using hierarchical cluster analysis as previously described [65], [66].

Not exact matches

However, microarray analysis is only capable of measuring the status of known transcripts, and expression of low - abundance mRNAs is often not detected by the hybridization - based approach.

Here we show that hybridization capture on microarrays can successfully recover more than a megabase of target regions from Neandertal DNA even in the presence of ~ 99.8 % microbial DNA.

The Research Histology Core Laboratory includes specialized equipment for immunohistochemistry, fluorescent in situ hybridization FISH, laser capture microdissection, and tissue microarray construction.

The high resolution that is achieved by these techniques, particularly by microarray technologies such as array comparative genomic hybridization, is blurring the traditional distinction between cytogenetics and molecular biology.

Compared with RNAseq we found that microarrays suffer from high levels of noise, possibly due to non-specific hybridization, and reach saturation with highly expressed genes [42].

Keywords: Gene expression, High performance liquid chromatography, Mass spectrometry, Microarray Analysis, Nucleic acid hybridization, Polymerase chain reaction - quantitative, Purification

Geographical location of the populations sampled for the transcriptome (red circles) and DNA hybridization on microarrays datasets (black circles).

Chromosomal position of the genomic regions targeted with the hybridization capture on microarrays.

We obtained polymorphism and divergence data from six individuals for each of the rabbit subspecies using Illumina sequencing of genomic regions enriched by DNA hybridization on microarrays [60].

We also describe a method for the bulk testing of the hybridization characteristics of chromosome - specific clones spotted on microarrays by use of DNA amplified from flow - sorted chromosomes.

ViroChip microarray, PCR, fluorescence in situ hybridization (FISH), serological, and deep sequencing analyses were performed in 4 different laboratories (Fig. 1).

We assessed the ability of a commercial DNA microarray to characterize bovine Shiga toxin - producing Escherichia coli (STEC) isolates and evaluated the results using in silico hybridization of the microarray probes within whole genome sequencing scaffolds.

Methylation profiles covering the promoter regions of 20 000 genes and 400 microRNAs were generated using MeDIP followed by hybridization to microarrays.