He has helped generate and interpret mountains of data through
microbial gene sequencing efforts, showing how microbes influence human well - being.
«I think it's important [for journals] to be discussing this, but I think it's unrealistic to think that journals can and should play the role of the sole enforcer,» says Claire Fraser, director of The Institute for Genomic Research in Rockville, Maryland, which posts a range of
microbial gene sequences online.
Not exact matches
Using metabolic function analyses of identified
genes, we compared our human genome with the average content of previously
sequenced microbial genomes.
We examined 13,355 prokaryotic ribosomal RNA
gene sequences from multiple colonic mucosal sites and feces of healthy subjects to improve our understanding of gut
microbial diversity.
We analyzed 7.2 terabases of metagenomic data from 243 Tara Oceans samples from 68 locations in epipelagic and mesopelagic waters across the globe to generate an ocean
microbial reference
gene catalog with > 40 million nonredundant, mostly novel
sequences from viruses, prokaryotes, and picoeukaryotes.
After six to eight weeks, the
microbial content of the mice's feces were analyzed by 16S ribosomal RNA
gene sequencing, which showed that approximately 20 kinds of oral bacteria were present in these mice.
Not only did the
gene sequencing show several bacteria could break down aromatic hydrocarbons, it illuminated how various species worked together to maximize the genetic potential of the whole
microbial community.
Thanks to ever - evolving
gene -
sequencing technology, scientists can now use
microbial RNA to identify organisms.
Collaborators at Human Longevity, Inc.
sequenced the
microbial genes extracted from each participant's stool sample and used that information to determine which species were living where, and the relative abundance of each.
The genome contains 3 to 4 times more DNA than other
microbial genomes
sequenced so far, and it's chock full of useful
genes with relatively little useless DNA in the way.
Much of what they
sequenced turned out to be
microbial contaminants, as expected, but 6 percent was cave bear nuclear DNA, including bits of 21
genes.
«The development of metagenomic
sequencing of the total DNA in a
microbial sample from the human body has allowed us to estimate the abundance of specific microbes and
microbial genes.
For instance, the Human Microbiome Project (HMP)(Turnbaugh et al, 2007; Peterson et al, 2009; Huttenhower et al, 2012) and MetaHIT (Qin et al, 2010) have generated maps of bacterial species abundances throughout the human body, reference genomes, and catalogs of more than 100 million
microbial genes assembled from shotgun
sequencing of in vivo communities.
$ 2,500 «Beyond bacteria» Deeper shotgun metagenome and virome characterization of one sample, plus additional marker
gene sequencing (16S rRNA, 18S rRNA and ITS, for you
sequencing wonks) to characterize not just the bacteria but also the viruses,
microbial eukaryotes (like giardia), and fungi in your gut.
We detected the
microbial communities of samples by usinghigh - throughput
sequencing of 16S rRNA
genes.
Following the isolation and purification of
microbial DNA from all samples, we used community fingerprinting (ARISA) and high - throughput
sequencing of the 16s ribosomal RNA
gene (V1 - V3) to describe the
microbial community structure in each group.
Professor Segal's research has two major directions 1)
Gene regulation — using quantitative and computational models to understand how DNA
sequence variation among human individuals generates phenotypic diversity 2) Microbiome and Nutrition — understanding how the
microbial composition of individuals affect their physiology and health.
In the lead profile, Jansson represented those researchers investigating such diseases by using omics technologies - uncovering the DNA
sequences, expressed
genes, and metabolite signatures used to reveal links to
microbial functions in the gut.
One question in analyzing
microbial communities by 16S rRNA
gene pyrosequencing centers on whether the amplification and
sequencing methods result in recovery of
sequences in proportion to their representation in the original community [24].
VITCOMIC2: visualization tool for the phylogenetic composition of
microbial communities based on 16S rRNA
gene amplicons and metagenomic shotgun
sequencing — Hiroshi Mori — BMC Systems Biology
Metagenomics is the high - throughput
sequencing of
microbial community DNA extracts and, because it directly
sequences this DNA, it can be used to characterize changes in the
genes themselves and their function.
A team from the University of California - Berkeley, Lawrence Berkeley National Laboratory, PNNL, and Oak Ridge National Laboratory
sequenced nearly 150,000
genes from three
microbial communities in samples from a subsurface aquifer at a uranium mill tailings site in Rifle, Colorado.
Other uses of having a known link between multiple DNA
sequencing reads includes studies of structural variations in for instance cancer cells, and when linking functional
genes to taxonomic groups in
microbial communities.
Assigning 16S rRNA
gene sequences to operational taxonomic units (OTUs) allows
microbial ecologists to overcome the inconsistencies and biases within bacterial taxonomy and provides a strategy for clustering similar
sequences that do not have representatives in a reference database.