Purifying membrane protein and growing membrane protein
microcrystals of bacterial rhodopsins and terminal oxidases.
Shen et al. grew ordered arrays of
microcrystals of the ZIF - 8 metal - organic framework, in which zinc ions are bridged by 2 - methylimidazole linkers, inside a porous polystyrene template.
Not exact matches
Wei Liu, a TSRI staff scientist who was first author
of the study, said, «It's a big advantage that you don't have to harvest individual crystals — you can just load the whole gel - like sample with embedded
microcrystals in the injector and start collecting data.
Perhaps the best known example is insulin: insulin shots comprise the subcutaneous injection
of a suspension
of insulin
microcrystals which dissolve slowly to yield a steady and sustained delivery over time.
Unlike
microcrystals, for example, the tubules have complex forms — often observed abruptly changing direction to avoid intersecting each other, in a manner consistent with tunneling microbial behavior — and contain organic molecules associated with biological activity; the Ries tubules are also similar to fossil traces
of microbes found in volcanic glass.
The dynamics
of these
microcrystals were captured in exquisite detail when the world's most powerful X-ray laser at SLAC was trained on them.
The ASU team contributed to many aspects
of the study, which range from experimental planning to the application
of injector technology, growth and biophysical characterization
of the PYP
microcrystals and data evalution.
The PYP
microcrystals were perfectly matched to the optical absorption so that the entire crystal was undergoing dynamics, which in turn allows sensitive measurements
of the molecular changes by snapshot X-ray diffraction.
Long grooves in quartz
microcrystals appear to be fossils
of microbes» cells.
Here, we demonstrate that MISC is feasible at an X-ray free electron laser by studying the reaction
of M. tuberculosisß - lactamase
microcrystals with ceftriaxone antibiotic solution.